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The effect of sodium bicarbonate and validation of beckman coulter AU680 analyzers for measuring total carbon dioxide (TCO2) concentrations in horse serum

机译:碳酸氢钠的作用和贝克曼库尔特AU680分析仪用于测定马血清中总二氧化碳(TCO2)浓度的验证

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摘要

This study evaluated the usage of Beckman Coulter AU680 analyzers for measurement of TCO 2 in horse serum, and the effect of sodium bicarbonate administrations on serum TCO 2 levels in resting horses. Treatment of horses with sodium bicarbonate did not result in any adverse events. Mean TCO 2 concentration was significantly higher from 1 to 8 h in the sodium bicarbonate‐treated horses compared to the untreated controls. Within an hour, administration of sodium bicarbonate increased the TCO 2 level from 31.5 ± −2.5 (SD) to 34.0 ± 2.65 (SD) mmol/L and at 2–8 h post‐administration, the TCO 2 level was above the 36 mmol/L cut‐off level. In all quality control analysis of Australian standard by Beckman Coulter AU680 analyzer, the instrument slightly over estimated the TCO 2 level but the values were in close agreement with mean TCO 2 level being 38.03 with ± 0.87 mmol/L (SD). Expanded uncertainty was calculated using different levels of confidence interval. Based on 99.5% confidence interval using 0.805% expanded uncertainty using mean measured concentration of 38.05 mmol/L, it was estimated that any race samples TCO 2 level higher than 38.5 mmol/L will be indicative of sodium bicarbonate administration using Beckman Coulter AU680 analyzer in Louisiana.
机译:这项研究评估了Beckman Coulter AU680分析仪在马血清中TCO 2的测量中的应用,以及碳酸氢钠对静息马血清TCO 2水平的影响。用碳酸氢钠治疗马匹未引起任何不良事件。与未处理的对照组相比,碳酸氢钠处理的马在1至8小时内的平均TCO 2浓度明显更高。在一个小时内,施用碳酸氢钠使TCO 2水平从31.5±-2.5(SD)升高至34.0±2.65(SD)mmol / L,并且在给药后2-8小时,TCO 2水平高于36 mmol / L截止水平。在使用贝克曼库尔特AU680分析仪对澳大利亚标准进行的所有质量控制分析中,该仪器略高于估计的TCO 2水平,但该值与TCO 2平均水平为38.03,±0.87 mmol / L(SD)密切相关。使用不同级别的置信区间计算扩展的不确定性。基于99.5%的置信区间,使用38.05 mmol / L的平均测得浓度的0.805%扩展不确定性,估计任何种族样品的TCO 2水平高于38.5 mmol / L都将表明使用Beckman Coulter AU680分析仪进行碳酸氢钠管理路易斯安那州。

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