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首页> 美国卫生研究院文献>AoB Plants >Sustainable production of azadirachtin from differentiated in vitro cell lines of neem (Azadirachta indica)
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Sustainable production of azadirachtin from differentiated in vitro cell lines of neem (Azadirachta indica)

机译：从印ne分化的体外细胞系中可持续生产印za素（印za素）

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摘要

Azadirachtin has high industrial demand due to its immediate application as an ecofriendly, biodegradable biopesticide and also due to its various other significant bioactivities. To date, the only commercially feasible way to produce azadirachtin is extraction from seeds, but their availability is very limited as the tree flowers only once a year and only one-third of the fruits are collected due to operational problems. Further, due to the strict out-breeding nature of the plant, the seeds are highly heterozygous, resulting in inconsistent metabolite production. Therefore, in the present study, to achieve sustainable production of azadirachtin, dedifferentiated and redifferentiated calli derived from various explants of neem—zygotic embryo, leaf and ovary—were investigated for their potential to biosynthesize azadirachtin. High-performance liquid chromatography analysis of the in vitro cell lines showed the presence of azadirachtin in all the samples tested, the content of which in cultured cells varied with explant source and cell differentiation response. The presence of azadirachtin in samples was further confirmed by positive electrospray ionization mass spectroscopy. The zygotic embryo cultures of neem accumulated much higher amounts of azadirachtin than leaf and ovary cultures. Furthermore, organized in vitro callus cultures (redifferentiated) supported higher azadirachtin biosynthesis, while unorganized callus cultures (dedifferentiated) supported the least. The maximum azadirachtin content of 2.33 mg g−1 dry weight was obtained from redifferentiated immature zygotic embryo cultures.
机译：印za素因其作为一种生态友好的，可生物降解的生物农药而立即应用以及其各种其他重要的生物活性而具有很高的工业需求。迄今为止，生产印苦ach子素的唯一商业上可行的方法是从种子中提取，但其可利用性非常有限，因为树花每年仅一次，并且由于操作问题仅收集了三分之一的果实。此外，由于植物的严格繁殖特性，种子是高度杂合的，导致代谢产物的产生不一致。因此，在本研究中，为了实现印za素的可持续生产，研究了从印of的各种外植体（合子，叶和卵巢）衍生的去分化和再分化的愈伤组织的生物合成印za素的潜力。体外细胞系的高效液相色谱分析显示，在所有测试样品中均存在印za素，培养物中细胞中印za素的含量随外植体来源和细胞分化反应而变化。通过正电喷雾电离质谱进一步证实了样品中印za素的存在。印em的合子胚培养物比叶和卵巢培养物积累的印za素含量高得多。此外，有组织的体外愈伤组织培养物（再分化）支持更高的印za素生物合成，而无组织的愈伤组织培养物（去分化）支持最少。从再分化的未成熟合子胚培养物中获得的最大印za素含量为2.33 mg g -1 干重。

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