• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Callus and azadirachtin related limonoids production through in vitro culture of neem (Azadirachta indica A. Juss)
【24h】

Callus and azadirachtin related limonoids production through in vitro culture of neem (Azadirachta indica A. Juss)

机译：愈伤组织和Azadirachtin相关的脂蛋白通过雷中的体外培养（Azadirachta indica A. Juss）

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A protocol was established for the induction of callus and suspension cultures for azadirachtin production from neem explants. Different concentrations and combinations of plant growth regulators (2,4-D, NAA, IAA and BAP) were supplemented in MS medium. Immature flowers, nodular stem sections, leaves immature embryos and mature seeds were used as explants.?The highest callus development (78%) was observed when immature flowers were inoculated on MS basal medium with addition of 1.0 mg/l 2,4-D, 1.0 mg/l?BAP, 0.2 mg/l NAA and 3% sucrose. The azadirachtin containing liminoids were determined from calli obtained from different explants. Effect of sucrose, glucose, NH4NO3, KNO3?and urea on cell suspension cultures and azadirachtin contents were also investigated. The dry cell weight and azadirachtin contents increased to?373.1 and 359.2?mg/ 50 ml when 0.25 and 0.5 g/l NH4NO3?was added in MS liquid media and supplemented with 1.0 mg/l2,4-D, 0.2 mg/l BAP and 3% sucrose.
机译：建立了诱导愈伤组织愈伤组织和悬浮培养物的愈伤组织和悬浮蛋白从Neem外来植物的产生。在MS培养基中补充了不同浓度和植物生长调节剂（2,4-D，NAA，IAA和BAP）的组合。不成熟的花朵，结节茎切片，叶未成熟的胚胎和成熟种子被用作外植体。当在MS基础培养基上接种未成熟的花朵时，观察到最高的愈伤组织发育（78％），加入1.0mg / L 2,4-d ，1.0mg / l？Bap，0.2mg / L NAA和3％蔗糖。从不同外植体获得的Calli测定含有吸毒素的Azadirachtin。还研究了蔗糖，葡萄糖，NH4NO3，KNO3和尿素对细胞悬浮培养物和阿扎托查汀内容物的影响。当0.25和0.5g / L NH 4 NO 3中加入时，干细胞重量和Azadirachtin含量增加到373.1和359.2·mg / 50ml。在MS液体培养基中加入并补充有1.0mg / L2,4-D，0.2mg / L Bap和3％的蔗糖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号