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首页> 美国卫生研究院文献>Journal of Insect Science >Insecticidal Effect of Labramin a Lectin—Like Protein Isolated from Seeds of the Beach Apricot Tree Labramia bojeri on the Mediterranean Flour Moth Ephestia kuehniella
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Insecticidal Effect of Labramin a Lectin—Like Protein Isolated from Seeds of the Beach Apricot Tree Labramia bojeri on the Mediterranean Flour Moth Ephestia kuehniella

机译：拉布拉明（一种凝集素样蛋白）的杀虫效果该蛋白是从海滩杏树（Labramia bojeri）种子中分离出的一种凝集素对地中海面粉蛾（Ephestia kuehniella）

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The objective of this work was to study the insecticidal effect of labramin, a protein that shows lectin—like properties. Labramin was isolated from seeds of the Beach Apricot tree, Labramia bojeri A. DC ex Dubard (Ericales: Sapotaceae), and assessed against the development of the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), an important pest of stored products such as corn, wheat, rice, and flour. Results showed that labramin caused 90% larval mortality when incorporated in an artificial diet at a level of 1% (w/w). The presence of 0.25% labramin in the diet affected the larval and pupal developmental periods and the percentage of emerging adults. Treatments resulted in elevated levels of trypsin activity in midgut and fecal materials, indicating that labramin may have affected enzyme—regulatory mechanisms by perturbing peritrophic membranes in the midgut of is. kuehniella larvae. The results of dietary experiments with E. kuehniella larvae showed a reduced efficiency for the conversion of ingested and digested food, and an increase in approximate digestibility and metabolic cost. These findings suggest that labramin may hold promise as a control agent to engineer crop plants for insect resistance.
机译：这项工作的目的是研究拉巴兰的杀虫效果，拉巴兰是一种具有凝集素样特性的蛋白质。拉布拉明从海滩杏树的种子（Labramia bojeri A. DC ex Dubard）中分离得到（Ericales：Sapotaceae），并针对地中海粉蛾Ephestia kuehniella Zeller（鳞翅目：Pyralidae）的发育进行了评估。如玉米，小麦，大米和面粉。结果显示，当以1％（w / w）的水平添加到人工饮食中时，拉巴兰可导致90％的幼虫死亡率。饮食中存在0.25％拉巴林会影响幼虫和小and的发育期以及成年成年人的百分比。治疗导致中肠和粪便中胰蛋白酶活性水平升高，表明拉巴兰可能会通过干扰中肠的营养膜而影响酶的调控机制。库氏菌幼虫。用库氏肠杆菌幼虫进行的饮食实验结果表明，摄入和消化食物的转化效率降低，并且近似消化率和代谢成本增加。这些发现表明，拉巴兰可以作为控制农作物抗虫性的控制剂。

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