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首页> 美国卫生研究院文献>Journal of Insect Science >Tissue and Life Stage Specificity of Glutathione S-Transferase Expression in the Hessian Fly Mayetiola destructor: Implications for Resistance to Host Allelochemicals
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Tissue and Life Stage Specificity of Glutathione S-Transferase Expression in the Hessian Fly Mayetiola destructor: Implications for Resistance to Host Allelochemicals

机译：谷胱甘肽S-转移酶表达在黑森州的苍蝇Mayetiola破坏者的组织和生命周期特异性：对宿主化感物质的抗性的影响。

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摘要

Two new Delta and Sigma glutathione S-transferases (GSTs) in the Hessian fly, Mayetiola destructor (Diptera: Cecidomyiidae), were characterized and transcription profiles described. The deduced amino acid sequences for the two M. destructor Delta GSTs (MdesGST-1 and MdesGST-3) showed high similarity with other insect Delta GSTs including the conserved catalytic serine residue. The deduced amino acid sequence for the M. destructor Sigma GST (MdesGST-2) showed high similarity with other insect Sigma GSTs including the conserved glutathione and substrate binding sites. Quantitative tissue expression analysis showed that mRNA levels for MdesGST-1 were predominant in fat body, whereas for MdesGST-2 and MdesGST-3 expression was predominant in the midgut. Temporal expression during development showed peak mRNA levels for MdesGST-1 during larval development, but in the pupal stage for MdesGST-2. MdesGST-3 showed a constitutive expression pattern throughout development. M. destructor feeds on wheat, and expression analysis after feeding indicated that mRNA levels for MdesGST-1 were significantly higher in incompatible interactions in which larvae fed on resistant wheat, while MdesGST-3 was significantly higher in compatible interactions when larvae fed on susceptible wheat. MdesGST-2 showed an equivalent expression pattern during both interactions. These results suggest that the M. destructor Delta GSTs are important in detoxifying wheat allelochemicals during feeding, while Sigma GST participates in metabolism of endogenous substrates.
机译：表征了黑森州蝇中的两个新的Delta和Sigma谷胱甘肽S-转移酶（GSTs），Mayetiola destructor（Diptera：Cecidomyiidae），并描述了转录谱。推导的两个解构分枝杆菌GST（MdesGST-1和MdesGST-3）的氨基酸序列与包括保守的催化丝氨酸残基在内的其他昆虫Delta GST显示出高度相似性。推断的解构支原体Sigma GST（MdesGST-2）的氨基酸序列与其他昆虫Sigma GST包括保守的谷胱甘肽和底物结合位点显示出高度相似性。定量组织表达分析表明，MdesGST-1的mRNA水平在脂肪体中占主导地位，而MdesGST-2和MdesGST-3的mRNA水平在中肠中占主导地位。发育过程中的时间表达表明，幼虫发育过程中MdesGST-1的mRNA水平达到峰值，而MdesGST-2处于the期。 MdesGST-3在整个发育过程中均显示出组成型表达模式。 M. destructor饲喂小麦，饲喂后的表达分析表明，以抗性小麦为食的幼虫在不相容相互作用中，MdesGST-1的mRNA水平显着较高，而以易感小麦为食的幼虫在相容性相互作用中，MdesGST-3的mRNA水平显着较高。 。 MdesGST-2在两种相互作用中均显示出等效的表达模式。这些结果表明，M。destructor Delta GST在饲喂过程中对小麦化感物质的解毒中很重要，而Sigma GST参与内源性底物的代谢。

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