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首页> 美国卫生研究院文献>3 Biotech >Genetic diversity among some canola cultivars as revealed by RAPD SSR and AFLP analyses
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Genetic diversity among some canola cultivars as revealed by RAPD SSR and AFLP analyses

机译：RAPDSSR和AFLP分析显示某些油菜品种的遗传多样性

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摘要

To assess the genetic diversity among four canola cultivars (namely, Serw-3, Serw-4, Misser L-16 and Semu 249), random amplified polymorphic DNA (RAPD), simple sequence repeat polymorphism (SSR) and amplified fragment length polymorphism (AFLP) analyses were performed. The data indicated that all of the three molecular markers gave different levels of polymorphism. A total of 118, 31 and 338 markers that show 61, 67.7 and 81 % polymorphism percentages were resulted from the RAPD, SSR and AFLP analyses, respectively. Based on the data obtained the three markers can be used to differentiate between the four canola cultivars. The genotype-specific markers were determined, 18 out of the 72 polymorphic RAPD markers generated were found to be genotype-specific (25 %). The highest number of RAPD specific markers was scored for Semu 249 (15 markers), while Serw-4 scored two markers. On the other hand, Serw-3 scored one marker. The cultivar Semu 249 scored the highest number of unique AFLP markers, giving 57 unique markers, followed by Misser L-16 which was characterized by 40 unique AFLP markers, then Serw-3 giving 31 unique markers. While Serw-4 was characterized by the lowest number producing 14 unique positive markers. The dendrogram built on the basis of combined data from RAPD, SSR and AFLP analysis represents the genetic distances among the four canola cultivars. Understanding the genetic variability among the current canola cultivars opens up a possibility for developing a molecular genetic map that will lead to the application of marker-assisted selection tools in genetic improvement of canola.
机译：为了评估四个油菜品种（即Serw-3，Servw-4，Misser L-16和Semu 249），随机扩增多态性DNA（RAPD），简单序列重复多态性（SSR）和扩增片段长度多态性（进行了AFLP）分析。数据表明所有三种分子标记均具有不同水平的多态性。通过RAPD，SSR和AFLP分析分别得出了分别显示61％，67.7％和81％多态性百分比的总共118、31和338个标记。基于获得的数据，可以使用三种标记物来区分四种低芥酸菜子品种。确定了基因型特异性标记，在生成的72个多态性RAPD标记中，有18种是基因型特异性的（25％）。最高的RAPD特异性标记是Semu 249（15个标记），而Serw-4则是两个标记。另一方面，Serw-3得分了一个标记。 Semu 249品种获得了最高数量的独特AFLP标记，给出了57个独特标记，其次是Misser L-16，其特征在于40个独特AFLP标记，然后是Serw-3获得了31个独特标记。 Serw-4的特征是产生14个独特阳性标记的最低数量。基于RAPD，SSR和AFLP分析的组合数据构建的树状图代表了四个油菜品种之间的遗传距离。了解当前双低油菜籽品种之间的遗传变异性，为开发分子遗传图谱提供了可能性，该分子图谱将导致标记辅助选择工具在双低油菜籽遗传改良中的应用。

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