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首页> 外文期刊>African Journal of Biotechnology >Inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) analyses of genetic diversity in Mehr cotton cultivar and its crossing progenies
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Inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) analyses of genetic diversity in Mehr cotton cultivar and its crossing progenies

机译：间简单重复序列（ISSR）和随机扩增多态性DNA（RAPD）分析Mehr棉花品种及其杂交后代的遗传多样性

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Cotton is cultivated in Iran with diploid and tetraploid forms and hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. This present study considered the molecular genetic diversity in Mehr (Gossypium hirsutum) cotton cultivar and its crossing progenies. 21 of 30?random amplified polymorphic DNA (RAPD) primers produced 220 reproducible bands with average of 10.47 bands per primer and 80.12% of polymorphism. OPR02 primer showed the highest number of effective allele (Ne), Shannon index (I) and genetic diversity (H). Some of the cultivars had specific bands, for example the F1 progeny of Mehr X No. 200, Mehr parental genotype and Mehr X Belilzovar hybrid genotype. Results show that some RAPD bands were present in the F1 progenies, but absent in the parental genotypes.? Some others were present in the parental genotypes, but were absent in their hybrids. The highest values of genetic diversity in RAPD markers were obtained in Mehr X Sindose and Mehr X Belilzovar hybrids. Nine of ten?inter simple sequence repeats?(ISSR) primers used produced 113 reproducible bands with average of 54.35% polymorphism. UBC834 locus revealed the highest number of Ne, I and H values. Also, some ISSR bands occurred only in the parental genotype while some others occurred only in the hybrid genotypes. The highest values of genetic diversity in ISSR markers were obtained in Mehr X Sindose and Mehr X Belilzovar hybrids.
机译：在伊朗以二倍体和四倍体形式种植棉花，杂交是增加遗传多样性并在该作物上获得新的优良品种的一种手段。本研究考虑了棉（Mehr（Gossypium hirsutum）棉）品种及其杂交后代的分子遗传多样性。 30个随机扩增的多态性DNA（RAPD）引物中有21个产生220条可复制的条带，平均每个引物具有10.47条带，且多态性为80.12％。 OPR02引物显示有效等位基因（Ne），香农指数（I）和遗传多样性（H）最高。一些品种具有特定条带，例如Mehr X No. 200的F1子代，Mehr亲本基因型和Mehr X Belilzovar杂种基因型。结果表明，F1后代中存在一些RAPD条带，而亲本基因型中不存在。亲本基因型中存在其他一些，但在其杂种中不存在。在Mehr X Sindose和Mehr X Belilzovar杂种中获得了RAPD标记中遗传多样性的最高值。十个“内部简单序列重复序列”（ISSR）引物中的九个产生了113条可再现的条带，平均多态性为54.35％。 UBC834基因座显示了最高的Ne，I和H值。另外，某些ISSR条带仅在亲本基因型中出现，而另一些仅在杂种基因型中出现。在Mehr X Sindose和Mehr X Belilzovar杂种中获得了ISSR标记中遗传多样性的最高值。

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