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首页> 外文会议>International Conference on Environment Science and Biotechnology >Genetic Diversity within and among Populations of Acarus siro L. Revealed by Inter Simple Sequence Repeats (ISSR)
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Genetic Diversity within and among Populations of Acarus siro L. Revealed by Inter Simple Sequence Repeats (ISSR)

机译：痤疮Siro L中的遗传多样性和胰岛癌的群体。通过简单的序列重复揭示（ISSR）

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摘要

Acarus siro L., a common acaroid mite in China, is one of the main pest mites damaging the stored products, economic crops and edible fungi. The mite is widely studied due to its economic importance. In this paper, the genetic structure and genetic diversity of A. siro in four geographic populations were studied by using ISSR marker. 18 primers were selected for their ability to produce clear and reproducible patterns of polymorphic bands. Those primers were then used for analysing genetic diversity within and among populations of A. siro collected in Nanchang city, Jiujiang city in liangxi province and Hefei city, Caohu city in Anhui province (NC, JJ, HF, CH). A total of 136 DNA bands were yielded, 107 of which were polymorphic and PPB was 78.68%. The levels of genetic diversity of four population could be ordered in abundance as IJ>NO HF>CH and the JJ population was the highest level. The value of Gst was 0.6283, indicating high level of genetic differentiation among populations. Nei's genetic diversity analysis showed that the level of gene flow (Nm=0.3717) was lower than that among populations (Nm=0.29). Dendrogram constructed using the UPMGA method and Nei's unbias genetic distance showed the same location could be clustered together to form a specific clade. Geographic difference caused great differentiation. Moreover, there was no significant correlation between the genetic distance and geographic distance, indicating that geographic isolation was not the main factors inducing genetic difference.
机译：Acarus Siro L.是中国常见的Acaroid螨虫，是害虫螨虫的主要害虫之一，损坏了存储的产品，经济作物和食用菌。由于其经济意义，螨虫被广泛研究。本文通过使用ISSR标记研究了四种地理群中A的遗传结构和遗传多样性。选择18个引物，用于它们产生多态带的透明和可再现模式的能力。然后将这些引物用于分析南昌市九江市江西省九江市和安徽省漕湖市合肥市收集的遗传多样性遗传多样性（NC，JJ，HF，CH）。总共产生136个DNA带，其中107个是多态，PPB为78.68％。四个人群的遗传多样性可以在丰富的情况下订购，因为IJ>否> CH和JJ人口是最高水平。 GST的值为0.6283，表明人群中高水平的遗传分化。 Nei的遗传多样性分析表明，基因流量（Nm = 0.3717）的水平低于群体（nm = 0.29）。使用UPMGA方法构造的树木图和NEI的遗传距离显示相同的位置可以聚集在一起以形成特定的疏水板。地理差异引起了极大的差异化。此外，遗传距离和地理距离之间没有显着的相关性，表明地理分离不是诱导遗传差异的主要因素。

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