首页> 美国卫生研究院文献>Applications in Plant Sciences >Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology1
【2h】

Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology1

机译:序列相关的扩增多态性(SRAP)标记:植物分子生物学研究的潜在资源1

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

In the past few decades, many investigations in the field of plant biology have employed selectively neutral, multilocus, dominant markers such as inter-simple sequence repeat (ISSR), random-amplified polymorphic DNA (RAPD), and amplified fragment length polymorphism (AFLP) to address hypotheses at lower taxonomic levels. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. These markers have proven to be robust and highly variable, on par with AFLP, and are attained through a significantly less technically demanding process. SRAP markers have been used primarily for agronomic and horticultural purposes, developing quantitative trait loci in advanced hybrids and assessing genetic diversity of large germplasm collections. Here, we suggest that SRAP markers should be employed for research addressing hypotheses in plant systematics, biogeography, conservation, ecology, and beyond. We provide an overview of the SRAP literature to date, review descriptive statistics of SRAP markers in a subset of 171 publications, and present relevant case studies to demonstrate the applicability of SRAP markers to the diverse field of plant biology. Results of these selected works indicate that SRAP markers have the potential to enhance the current suite of molecular tools in a diversity of fields by providing an easy-to-use, highly variable marker with inherent biological significance.
机译:在过去的几十年中,植物生物学领域的许多研究都采用了选择性的中性,多位点,显性标记,例如简单序列间重复(ISSR),随机扩增多态性DNA(RAPD)和扩增片段长度多态性(AFLP)。 )以在较低的分类标准下处理假设。最近,已经开发了与序列相关的扩增多态性(SRAP)标记,该标记用于通过靶向开放阅读框的引物扩增DNA的编码区。与AFLP相比,这些标记已被证明是可靠且高度可变的,并且通过对技术要求不高的工艺来获得。 SRAP标记主要用于农艺和园艺目的,在高级杂种中建立定量性状基因座,并评估大型种质资源的遗传多样性。在这里,我们建议将SRAP标记用于研究植物系统学,生物地理学,自然保护,生态学以及其他方面的假设。我们提供了迄今为止SRAP文献的概述,回顾了171种出版物中SRAP标记的描述性统计数据,并提供了相关的案例研究,以证明SRAP标记在植物生物学的各个领域的适用性。这些选定作品的结果表明,SRAP标记物具有提供具有内在生物学意义的易于使用的,高度可变的标记物的潜力,从而可以在多种领域中增强当前的分子工具套件。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号