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Development of microsatellite loci for the riparian tree species Melaleuca argentea (Myrtaceae) using 454 sequencing

机译:利用454测序技术开发河岸树木白千层(Myrtaceae)微卫星基因座

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摘要

• Premise of the study: Microsatellite primers were developed for Melaleuca argentea (Myrtaceae) to evaluate genetic diversity and population genetic structure of this broadly distributed northern Australian riparian tree species.• Methods and Results: 454 GS-FLX shotgun sequencing was used to obtain 5860 sequences containing putative microsatellite motifs. Two multiplex PCRs were optimized to genotype 11 polymorphic microsatellite loci. These loci were screened for variation in individuals from two populations in the Pilbara region, northwestern Western Australia. Overall, observed heterozygosities ranged from 0.27 to 0.86 (mean: 0.52) and the number of alleles per locus ranged from two to 13 (average: 4.3).• Conclusions: These microsatellite loci will be useful in future studies of the evolutionary history and population and spatial genetic structure in M. argentea, and inform the development of seed sourcing strategies for the species.
机译:•研究前提:为白千层(Myrtaceae)开发了微卫星引物,以评估这种分布广泛的北澳大利亚河岸树种的遗传多样性和种群遗传结构。•方法和结果:使用454 GS-FLX shot弹枪测序获得5860包含推定的微卫星基序的序列。优化了两个多重PCR以适应基因型11多态微卫星基因座。对这些基因座筛选了西澳大利亚州西北部Pilbara地区两个种群的个体差异。总体而言,观察到的杂合度范围为0.27至0.86(平均值:0.52),每个位点的等位基因数量范围为2至13(平均:4.3)。•结论:这些微卫星基因座将在未来进化史和种群研究中有用和M. argentea的空间遗传结构,并为该物种的种子采购策略的发展提供信息。

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