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首页> 美国卫生研究院文献>Nutrients >A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers
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A Novel Biomimetic Tool for Assessing Vitamin K Status Based on Molecularly Imprinted Polymers

机译：一种基于分子印迹聚合物的新型仿生工具用于评估维生素K的状态

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摘要

Vitamin K was originally discovered as a cofactor required to activate clotting factors and has recently been shown to play a key role in the regulation of soft tissue calcification. This property of vitamin K has led to an increased interest in novel methods for accurate vitamin K detection. Molecularly Imprinted Polymers (MIPs) could offer a solution, as they have been used as synthetic receptors in a large variety of biomimetic sensors for the detection of similar molecules over the past few decades, because of their robust nature and remarkable selectivity. In this article, the authors introduce a novel imprinting approach to create a MIP that is able to selectively rebind vitamin K1. As the native structure of the vitamin does not allow for imprinting, an alternative imprinting strategy was developed, using the synthetic compound menadione (vitamin K3) as a template. Target rebinding was analyzed by means of UV-visible (UV-VIS) spectroscopy and two custom-made thermal readout techniques. This analysis reveals that the MIP-based sensor reacts to an increasing concentration of both menadione and vitamin K1. The Limit of Detection (LoD) for both compounds was established at 700 nM for the Heat Transfer Method (HTM), while the optimized readout approach, Thermal Wave Transport Analysis (TWTA), displayed an increased sensitivity with a LoD of 200 nM. The sensor seems to react to a lesser extent to Vitamin E, the analogue under study. To further demonstrate its potential application in biochemical research, the sensor was used to measure the absorption of vitamin K in blood serum after taking vitamin K supplements. By employing a gradual enrichment strategy, the sensor was able to detect the difference between baseline and peak absorption samples and was able to quantify the vitamin K concentration in good agreement with a validation experiment using High-Performance Liquid Chromatography (HPLC). In this way, the authors provide a first proof of principle for a low-cost, straightforward, and label-free vitamin K sensor.
机译：最初发现维生素K是激活凝血因子所需的辅助因子，最近发现它在调节软组织钙化中起关键作用。维生素K的这种特性导致人们对用于准确检测维生素K的新方法的兴趣日益增加。分子印迹聚合物（MIP）可以提供一种解决方案，因为它们在过去几十年中已被用作多种仿生传感器的合成受体，用于检测相似分子，因为它们具有稳健的性质和出色的选择性。在本文中，作者介绍了一种新颖的印迹方法，以创建能够选择性地重新结合维生素K1的MIP。由于维生素的天然结构不允许印迹，因此使用合成化合物甲萘醌（维生素K3）作为模板，开发了另一种印迹策略。通过UV-可见（UV-VIS）光谱和两种定制的热读出技术分析靶标的结合。该分析表明，基于MIP的传感器对甲萘醌和维生素K1浓度的增加有反应。对于传热方法（HTM），这两种化合物的检出限（LoD）均设置为700 nM，而优化的读出方法，热波传输分析（TWTA）显示出更高的灵敏度，LoD为200 nM。传感器似乎对正在研究的类似物维生素E的反应程度较小。为了进一步证明其在生化研究中的潜在应用，该传感器用于在服用维生素K补充剂后测量血清中维生素K的吸收。通过采用逐步富集策略，该传感器能够检测基线和峰值吸收样品之间的差异，并且能够与使用高效液相色谱（HPLC）进行的验证实验很好地量化维生素K的浓度。通过这种方式，作者为低成本，简单明了且无标签的维生素K传感器提供了原理的第一个证明。

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