• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Microbially Enhanced Oil Recovery by Alkylbenzene-Oxidizing Nitrate-Reducing Bacteria
【2h】

Microbially Enhanced Oil Recovery by Alkylbenzene-Oxidizing Nitrate-Reducing Bacteria

机译：烷基苯氧化减少硝酸盐细菌的微生物提高石油采收率

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microbially enhanced oil recovery (MEOR) of heavy oil and bitumen is challenging because light hydrocarbons, which can feed resident microbial communities are present in low concentrations, if at all. We have recently shown that increasing the toluene concentration of heavy oil by aqueous injection followed by injection of nitrate boosts the activity of toluene-oxidizing nitrate-reducing bacteria in heavy oil-containing sand pack columns, giving production of residual oil in place (ROIP). In the current work we found that ethylbenzene is as effective as toluene. Microbial community analyses indicated Thauera and Pseudomonas to be main components of nitrate-containing batch and continuous cultures, regardless whether ethylbenzene or toluene was used as the electron donor. Biomass from batch cultures grown with heavy oil amended with ethylbenzene or toluene and nitrate or biomass from continuous cultures grown on ethylbenzene or toluene and nitrate had similar MEOR activity. Increasing the concentration of injected biomass from continuous cultures increased the fraction of ROIP recovered both in the absence and in the presence of nitrate. Nitrate increased the fraction of ROIP recovered by about 2-fold by increasing the concentration of biomass in the columns. Emulsification of oil by surface-adhering biomass and blocking of aqueous flow channels by oil emulsion droplets are proposed as a possible mechanism of hydrocarbon- and nitrate-mediated MEOR. Pure isolates Thauera sp. NS1 and Pseudomonas sp. NS2, which used both ethylbenzene and toluene, were obtained but did not offer improved MEOR compared to the use of batch and continuous cultures.
机译：微生物提高重油和沥青的采收率（MEOR）颇具挑战性，因为可以为居民微生物群落供食的轻质烃（如果有的话）浓度很低。我们最近发现，通过水相注入再注入硝酸盐来增加重油中的甲苯浓度，可以提高含重油的沙包塔中甲苯氧化减少硝酸盐的细菌的活性，从而就地产生残余油（ROIP） 。在当前的工作中，我们发现乙苯与甲苯一样有效。微生物群落分析表明，无论乙基苯或甲苯用作电子供体，Thauera和假单胞菌都是含硝酸盐的分批培养和连续培养的主要成分。用重油加乙苯或甲苯和硝酸盐改性的分批培养物的生物质，或用乙苯或甲苯和硝酸盐生长的连续培养物的生物质具有相似的MEOR活性。在不存在硝酸盐和存在硝酸盐的情况下，从连续培养物中增加注入的生物质的浓度都会增加回收的ROIP的比例。硝酸盐通过增加柱中生物量的浓度使回收的ROIP分数增加了约2倍。通过表面粘附生物质进行油的乳化和通过油乳剂液滴阻塞水的流动通道被认为是烃和硝酸盐介导的MEOR的可能机理。纯分离株Thauera sp。 NS1和假单胞菌sp。获得了同时使用乙苯和甲苯的NS2，但与分批和连续培养相比，没有提供改进的MEOR。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号