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首页> 外文期刊>Petroleum Science and Technology >Studies on the Production of Biosurfactant for the Microbial Enhanced Oil Recovery by Using Bacteria Isolated from Oil Contaminated Wet Soil
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Studies on the Production of Biosurfactant for the Microbial Enhanced Oil Recovery by Using Bacteria Isolated from Oil Contaminated Wet Soil

机译：用油污湿土中分离的细菌生产微生物表面活性剂的研究

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There are obvious advantages of biosurfactants over chemical surfactants. The developing shortage of oil and rapid increase of oil prices is putting pressure on oil companies to recover as much oil as possible from the wells to sustain the oil economy. Therefore, there is a need to research some "super bugs," which can produce active and stable biosurfactants in good yields. Five bacterial strains presently isolated from the oil-contaminated soil were selected for the screening for biosurfactant production, via three different methods: surface tension measurements, drop-collapsing test, and emulsification index (EI24) test. Two thermophillic isolates coded as SGI and LFA were found to be the suitable candidates for biosurfactant production. In fact, the biosurfactant produced by the isolate SGI led to the reduction of surface tension up to 26 m/N/m; thus, SGI was selected for the further studies. Biosurfactant production by the thermophillic isolate SGI was found to be growth-associated in all conditions tested. Biosurfactant production using different cheaper carbon substrates was studied. The production of biosurfactant was also studied using isolate SGI, under different conditions of high temperature, NaCl concentration, pH, carbon source, and initial nitrogen concentration. The biosurfactant was found to produce a relatively stable emulsion with hydrocarbons at a wide range of pH. It was also found to be stable at various pH ranges (7.0-14.0) for SGI and was also found to be thermostable for 1 hr at 125 deg C, based on the value of surface tension. There is a wide array of further studies in the area of microbial enhanced oil recovery (MEOR) including further boosting the activity of the isolate by using adaptation, enrichment, and nutrient enrichment techniques.
机译：生物表面活性剂比化学表面活性剂具有明显的优势。日益严重的石油短缺和油价的迅速上涨，给石油公司施加了压力，要求它们从油井中回收尽可能多的石油以维持石油经济。因此，有必要研究一些“超级虫子”，它们可以以高产量产生活性和稳定的生物表面活性剂。通过三种不同的方法，从油污土壤中分离出了五种细菌菌株，用于筛选生物表面活性剂的生产：表面张力测量，滴落塌陷测试和乳化指数（EI24）测试。发现编码为SGI和LFA的两种嗜热菌株是生产生物表面活性剂的合适候选者。实际上，由分离物SGI产生的生物表面活性剂导致表面张力降低了26 m / N / m。因此，选择了SGI进行进一步研究。发现在所有测试条件下，嗜热分离物SGI的生物表面活性剂生产均与生长相关。研究了使用不同的廉价碳底物生产生物表面活性剂。还使用分离的SGI在高温，NaCl浓度，pH，碳源和初始氮浓度的不同条件下研究了生物表面活性剂的生产。发现该生物表面活性剂在较宽的pH范围内与烃产生相对稳定的乳液。基于表面张力的值，还发现它对于SGI在各种pH范围（7.0-14.0）下都是稳定的，并且还发现在125℃下可稳定1小时。在微生物提高油采收率（MEOR）领域有广泛的进一步研究，包括通过使用适应，富集和营养富集技术进一步提高分离物的活性。

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