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首页> 美国卫生研究院文献>Journal of Genetic Engineering Biotechnology >Identification of ISSR and RAPD markers linked to yield traits in bread wheat under normal and drought conditions
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Identification of ISSR and RAPD markers linked to yield traits in bread wheat under normal and drought conditions

机译：正常和干旱条件下与面包小麦产量性状相关的ISSR和RAPD标记的鉴定

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Genetic variability and identification of some molecular markers were studied in twenty promising lines of wheat using agronomic traits, ISSR (inter simple sequences repeats) and RAPD (random amplified polymorphic DNA) markers. Significant variation was evidenced in all agronomic traits. The lines proved to be superior to the check cultivar Sahel1 in yield and its component traits. Lines L2, L7 and L8 were the best in most yield component traits in both seasons. Moreover, Lines L2, L4, L5, L7 and L8 showed drought tolerance by which they displayed high performance in agronomic traits as well as a low drought susceptibility index. The percentage of polymorphism was 39.3% and 53.2% for ISSRs and RAPDs, respectively. UBC-881 belonged to penta-nucleotide repeat sequences (GGGTG) that produced the highest level of polymorphism, while UBC-846 belonged to di-nucleotide repeat sequences (CA) that produced the lowest level of polymorphism. Genetic similarities among wheat lines based on ISSR and RAPD markers ranged from 0.81 to 1.00 and from 0.86 to 0.98, respectively. There was a low average of PIC (polymorphism information content) values which were 0.10 (ISSR) and 0.15 (RAPD). The RAPD technique exhibited a higher marker index (MI = 0.69) compared to ISSR (MI = 0.43). There was insignificant correlation between ISSR and RAPD data (0.168, p > 0.05). There were two markers (UBC-881450bp and OPF-10540bp), on each of which two traits regressed significantly. The associated markers each explained a maximum regression of 18.92–34.95% of the total available variation for individual associated traits.
机译：利用农艺性状，ISSR（简单重复序列间）和RAPD（随机扩增多态性DNA）标记，在二十个很有希望的小麦品系中研究了遗传变异性和一些分子标记的鉴定。在所有农艺性状上均表现出显着差异。该品系在产量及其组成性状上均优于对照品种Sahel1。在两个季节中，L2，L7和L8系表现出最多的产量构成特征。此外，L2，L4，L5，L7和L8品系表现出耐旱性，通过它们表现出较高的农艺性状和较低的干旱敏感性指数。 ISSR和RAPD的多态性百分比分别为39.3％和53.2％。 UBC-881属于产生最高水平多态性的五核苷酸重复序列（GGGTG），而UBC-846属于产生最低水平多态性的二核苷酸重复序列（CA）。基于ISSR和RAPD标记的小麦品系之间的遗传相似性分别为0.81至1.00和0.86至0.98。 PIC（多态信息含量）值的平均值较低，分别为0.10（ISSR）和0.15（RAPD）。与ISSR（MI = 0.43）相比，RAPD技术显示出更高的标记指数（MI = 0.69）。 ISSR和RAPD数据之间无显着相关性（0.168，p> 0.05）。有两个标记（UBC-881450bp和OPF-10540bp），每个标记的两个性状显着退化。每个相关的标记都解释了单个相关性状的总可用变异的最大回归值为18.92–34.95％。

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