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SPEKcheck — fluorescence microscopy spectral visualisation and optimisation: a web application javascript library and data resource

机译:SPEKcheck —荧光显微镜光谱的可视化和优化:Web应用程序javascript库和数据资源

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摘要

Advanced fluorescence imaging methods require careful matching of excitation sources, dichroics, emission filters, detectors, and dyes to operate at their best. This complex task is often left to guesswork, preventing optimal dye:filter combinations, particularly for multicolour applications. To overcome this challenge we developed SPEKcheck, a web application to visualise the efficiency of the light path in a fluorescence microscope. The software reports values for the excitation efficiency of a dye, the collection efficiency of the emitted fluorescence, and a "brightness" score, allowing easy comparison between different fluorescent labels. It also displays a spectral plot of various elements in the configuration, enabling users to readily spot potential problems such as low efficiency excitation, emission, or high bleedthrough. It serves as an aid to exploring the performance of different dyes and filter sets.
机译:先进的荧光成像方法需要仔细匹配激发源,二向色镜,发射滤光片,检测器和染料,以使其发挥最佳性能。这项繁琐的任务通常任凭猜测,以防止最佳的染料:过滤器组合,尤其是在多色应用中。为了克服这一挑战,我们开发了SPEKcheck,这是一个用于在荧光显微镜中可视化光路效率的Web应用程序。该软件报告染料的激发效率,发射的荧光的收集效率和“亮度”得分的值,从而可以轻松比较不同的荧光标记。它还显示配置中各种元素的频谱图,使用户能够轻松发现潜在问题,例如低效率的激发,发射或高漏泄。它有助于探索不同染料和滤光片组的性能。

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