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Persistent expression of Cotesia plutellae bracovirus genes in parasitized host Plutella xylostella

机译:Cotesia plutellae bracovirus基因在寄生寄主小菜蛾中的持久表达

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摘要

Cotesia plutellae (= vestalis) bracovirus (CpBV) is symbiotic to an endoparasitoid wasp, C. plutellae, and plays crucial roles in parasitism against the diamondback moth, Plutella xylostella. CpBV virion genome consists of 35 circular DNAs encoding 157 putative open reading frames (ORFs). This study re-annotated 157 ORFs with update genome database and analyzed their gene expressions at early and late parasitic stages. Re-annotation has established 15 different viral gene families, to which 83 ORFs are assigned with remaining 74 hypothetical genes. Among 157 ORFs, 147 genes were expressed at early or late parasitic stages, among which 141 genes were expressed in both parasitic stages, indicating persistent nature of gene expression. Relative frequencies of different viral circles present in the ovarian lumen did not explain the expression variation of the viral ORFs. Furthermore, expression level of each viral gene was varied during parasitism along with host development. Highly up-regulated CpBV genes at early parasitic stage included BEN (BANP, E5R and NAC1), ELP (EP1-like protein), IkB (inhibitor kB), P494 (protein 494 kDa) family genes, while those at late stage were mostly hypothetical genes. Along with the viral gene expression, 362 host genes exhibited more than two fold changes in expression levels at early parasitic stage compared to nonparasitized host. At late stage, more number (1,858) of host genes was regulated. These results suggest that persistent expression of most CpBV genes may be necessary to regulate host physiological processes during C. plutellae parasitism.
机译:小菜蛾(Cotesia plutellae)等于内寄生蜂黄蜂C. plutellae的共生体,在对小菜蛾小菜蛾小菜蛾(Plutella xylostella)的寄生中起关键作用。 CpBV病毒体基因组由35个环状DNA组成,编码157个推定开放阅读框(ORF)。这项研究用更新的基因组数据库重新注释了157个ORF,并分析了它们在寄生虫早期和晚期的基因表达。重新注释已经建立了15个不同的病毒基因家族,其中83个ORF被分配了剩余的74个假设基因。在157个ORF中,在早期或晚期寄生虫阶段表达了147个基因,其中在两个寄生虫阶段都表达了141个基因,表明基因表达的持久性。卵巢腔中存在不同病毒圈的相对频率不能解释病毒ORF的表达变异。此外,在寄生过程中随着宿主的发展,每个病毒基因的表达水平也发生了变化。寄生虫早期高度上调的CpBV基因包括BEN(BANP,E5R和NAC1),ELP(EP1样蛋白),IkB(抑制剂kB),P494(蛋白494 kDa)家族基因,而晚期的大多数假设基因。与病毒基因表达一起,与非寄生虫宿主相比,362个宿主基因在寄生前期的表达水平表现出超过两倍的变化。在后期阶段,更多数目的宿主基因被调控(1,858)。这些结果表明,大多数CpBV基因的持续表达可能是调节小菜蛾寄生虫期间宿主生理过程所必需的。

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    Yonggyun Kim; Sunil Kumar;

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  • 年(卷),期 -1(13),7
  • 年度 -1
  • 页码 e0200663
  • 总页数 19
  • 原文格式 PDF
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