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首页> 美国卫生研究院文献>other >Use of the PCR-DGGE Method for the Analysis of the Bacterial Community Structure in Soil Treated With the Cephalosporin Antibiotic Cefuroxime and/or Inoculated With a Multidrug-Resistant Pseudomonas putida Strain MC1
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Use of the PCR-DGGE Method for the Analysis of the Bacterial Community Structure in Soil Treated With the Cephalosporin Antibiotic Cefuroxime and/or Inoculated With a Multidrug-Resistant Pseudomonas putida Strain MC1

机译：PCR-DGGE方法在分析用头孢菌素抗生素头孢呋辛和/或接种多药耐药恶臭假单胞菌MC1的土壤中细菌群落结构的分析

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The widespread use of cefuroxime (XM) has resulted in the increase in its concentration in hospital and domestic wastewaters. Due to the limited removal of antibiotics and antibiotic-resistant genes in conventional systems, the drugs enter the surface water and soils. Moreover, the introduction of XM and/or XM-resistant bacteria into soil may cause a significant modification of the biodiversity of soil bacterial communities. Therefore, the goal of this research was to assess the genetic diversity of a bacterial community in the cefuroxime (XM1 – 1 mg/kg and XM10 – 10 mg/kg) and/or antibiotic-resistant Pseudomonas putida strain MC1 (Ps – 1.6 × 107 cells/g)-treated soils as determined by the DGGE (denaturing gradient gel electrophoresis) method. The obtained data were also evaluated using a multivariate analysis and the resistance (RS)/resilience (RL) concept. Strain MC1 was isolated from raw sewage in the presence of XM and was resistant not only to this antibiotic but also to vancomycin, clindamycin and erythromycin. The DGGE patterns revealed that the XM10 and XM10+Ps treatments modified the composition of the bacterial community by the alteration of the DGGE profiles as well as a decline in the DGGE indices, in particular on days 30, 60, and 90. In turn, the XM1 and XM1+Ps or Ps treatments did not affect the values of richness and diversity of the soil bacteria members. A principal component analysis (PCA) also indicated that XM markedly changed the diversity of bacterial assemblages in the second part of the experiment. Moreover, there were differences in the RS/RL of the DGGE indices to the disturbances caused by XM and/or Ps. Considering the mean values of the RS index, the resistance was categorized in the following order: diversity (0.997) > evenness (0.993) > richness (0.970). The soil RL index was found to be negative, thus reflecting the progressing detrimental impact of XM on the genetic biodiversity of bacteria within the experiment. These results indicate that the introduction of XM at higher dosages into the soil environment may exert a potential risk for functioning of microorganism.
机译：头孢呋辛（XM）的广泛使用导致其在医院和生活污水中的浓度增加。由于常规系统中对抗生素和抗药性基因的去除受限，药物进入地表水和土壤。而且，向土壤中引入XM和/或抗XM的细菌可能引起土壤细菌群落生物多样性的显着改变。因此，本研究的目的是评估头孢呋辛（XM1 – 1 mg / kg和XM10 – 10 mg / kg）和/或抗药性假单胞菌恶臭假单胞菌MC1（Ps – 1.6×用DGGE（变性梯度凝胶电泳）法测定10 7 细胞/克。还使用多变量分析和电阻（RS）/弹性（RL）概念对获得的数据进行了评估。 MC1菌株是在XM存在下从原始污水中分离出来的，不仅对这种抗生素具有抗药性，而且对万古霉素，克林霉素和红霉素也有抗药性。 DGGE模式显示，XM10和XM10 + Ps处理通过改变DGGE谱图以及DGGE指数的下降，尤其是在第30、60和90天，改变了细菌群落的组成。 XM1和XM1 + Ps或Ps处理不会影响土壤细菌成员的丰富度和多样性。主成分分析（PCA）还表明，XM在实验的第二部分显着改变了细菌集合的多样性。此外，DGGE指数的RS / RL与由XM和/或Ps引起的干扰存在差异。考虑到RS指数的平均值，将电阻按以下顺序分类：多样性（0.997）>均匀度（0.993）>丰富度（0.970）。发现土壤RL指数为负，从而反映了XM对实验中细菌遗传生物多样性的不利影响。这些结果表明，将较高剂量的XM引入土壤环境可能会对微生物的功能产生潜在的风险。

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