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首页> 美国卫生研究院文献>other >Fungal Gene Mutation Analysis Elucidating Photoselective Enhancement of UV-C Disinfection Efficiency Toward Spoilage Agents on Fruit Surface
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Fungal Gene Mutation Analysis Elucidating Photoselective Enhancement of UV-C Disinfection Efficiency Toward Spoilage Agents on Fruit Surface

机译：真菌基因突变分析阐明了对水果表面变质剂的UV-C消毒效率的光选择性增强

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Short-wave ultraviolet (UV-C) treatment represents a potent, clean and safe substitute to chemical sanitizers for fresh fruit preservation. However, the dosage requirement for microbial disinfection may have negative effects on fruit quality. In this study, UV-C was found to be more efficient in killing spores of Botrytis cinerea in dark and red light conditions when compared to white and blue light. Loss of the blue light receptor gene Bcwcl1, a homolog of wc-1 in Neurospora crassa, led to hypersensitivity to UV-C in all light conditions tested. The expression of Bcuve1 and Bcphr1, which encode UV-damage endonuclease and photolyase, respectively, were strongly induced by white and blue light in a Bcwcl1-dependent manner. Gene mutation analyses of Bcuve1 and Bcphr1 indicated that they synergistically contribute to survival after UV-C treatment. In vivo assays showed that UV-C (1.0 kJ/m2) abolished decay in drop-inoculated fruit only if the UV-C treatment was followed by a dark period or red light, while in contrast, typical decay appeared on UV-C irradiated fruits exposed to white or blue light. In summary, blue light enhances UV-C resistance in B. cinerea by inducing expression of the UV damage repair-related enzymes, while the efficiency of UV-C application for fruit surface disinfection can be enhanced in dark or red light conditions; these principles seem to be well conserved among postharvest fungal pathogens.
机译：短波紫外线（UV-C）处理代表了化学消毒剂的有效，清洁和安全替代品，可用于保鲜水果。但是，微生物消毒的剂量要求可能会对果实品质产生负面影响。在这项研究中，与白蓝光相比，UV-C在暗和红光条件下更能有效杀死灰霉病菌的孢子。蓝光受体基因Bcwcl1（crus孢子虫中wc-1的同系物）的丢失导致在所有测试的光照条件下对UV-C过敏。白光和蓝光以Bcwcl1依赖性方式强烈诱导Bcuve1和Bcphr1的表达，它们分别编码紫外线损伤的核酸内切酶和光裂解酶。 Bcuve1和Bcphr1的基因突变分析表明，它们协同作用有助于UV-C处理后的存活。体内试验表明，仅当紫外线-C处理后经过暗期或红光照射时，紫外线-C（1.0 kJ / m 2 ）才能消除滴滴接种的果实中的腐烂，而相反，典型的衰变出现在暴露于白光或蓝光的UV-C照射的水果上。总而言之，蓝光通过诱导与紫外线损伤修复相关的酶的表达增强灰质芽孢杆菌对紫外线-C的抗性，而在暗光或红光条件下，紫外线-C应用于水果表面消毒的效率可以提高；这些原理在收获后的真菌病原体中似乎保存得很好。

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