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首页> 美国卫生研究院文献>Journal of Genomics >Whole Genome Sequence Analysis of an Alachlor and Endosulfan Degrading Micrococcus sp. strain 2385 Isolated from Ochlockonee River Florida
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Whole Genome Sequence Analysis of an Alachlor and Endosulfan Degrading Micrococcus sp. strain 2385 Isolated from Ochlockonee River Florida

机译：甲草胺和硫丹降解微球菌的全基因组序列分析。 2385株从佛罗里达州奥克洛克尼河分离

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摘要

We recently isolated Micrococcus sp. strain 2385 from Ochlockonee River, Florida and demonstrated potent biodegradative activity against two commonly used pesticides- alachlor [(2-chloro-2`,6`-diethylphenyl-N (methoxymethyl)acetanilide)] and endosulfan [(6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,3,4-benzo(e)di-oxathiepin-3-oxide], respectively. To further identify the repertoire of metabolic functions possessed by strain 2385, a draft genome sequence was obtained, assembled, annotated and analyzed. The genome sequence of Micrococcus sp. strain 2385 consisted of 1,460,461,440 bases which assembled into 175 contigs with an N50 contig length of 50,109 bases and a coverage of 600x. The genome size of this strain was estimated at 2,431,226 base pairs with a G+C content of 72.8 and a total number of 2,268 putative genes. RAST annotated a total of 340 subsystems in the genome of strain 2385 along with the presence of 2,177 coding sequences. A genome wide survey indicated that that strain 2385 harbors a plethora of genes to degrade other pollutants including caprolactam, PAHs (such as naphthalene), styrene, toluene and several chloroaromatic compounds.
机译：我们最近分离了Micrococcus sp。佛罗里达州奥克洛克尼河（Ochlockonee River）的2385菌株，对两种常用农药甲草胺[（2-chloro-2`，6`-二乙基苯基-N（甲氧基甲基）乙酰胺）]和硫丹[（6,7,8， [9,10,10-hexachloro-1,5,5a，6,9,9a-hexahydro-6,9methano-2,3,4-benzo（e）di-oxathiepin-3-oxide]分别用于进一步鉴定通过对2385菌株的代谢功能库的研究，获得了拟南芥基因组序列，进行了组装，注释和分析，其中Micrococcus sp。2385菌株的基因组序列由1,460,461,440个碱基组成，它们被组装成175个contig，N50 contig长度为50,109个碱基，该菌株的基因组大小估计为2,431,226个碱基对，G + C含量为72.8，推定基因总数为2,268，RAST标记了菌株2385的基因组中总共340个子系统。存在2177个编码序列。基因组范围内的调查表明2385株带有大量可以降解其他污染物的基因，包括己内酰胺，PAH（例如萘），苯乙烯，甲苯和几种氯代芳香族化合物。

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