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首页> 美国卫生研究院文献>PLoS Neglected Tropical Diseases >Proteomic Comparison of Entamoeba histolytica and Entamoeba dispar and the Role of E. histolytica Alcohol Dehydrogenase 3 in Virulence
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Proteomic Comparison of Entamoeba histolytica and Entamoeba dispar and the Role of E. histolytica Alcohol Dehydrogenase 3 in Virulence

机译：溶血性变形虫和变形虫的蛋白质组学比较以及溶酶性大肠杆菌脱氢酶3在毒力中的作用

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摘要

The protozoan intestinal parasite Entamoeba histolytica infects millions of people worldwide and is capable of causing amebic dysentery and amebic liver abscess. The closely related species Entamoeba dispar colonizes many more individuals, but this organism does not induce disease. To identify molecular differences between these two organisms that may account for their differential ability to cause disease in humans, we used two-dimensional gel-based (DIGE) proteomic analysis to compare whole cell lysates of E. histolytica and E. dispar. We observed 141 spots expressed at a substantially (>5-fold) higher level in E. histolytica HM-1∶IMSS than E. dispar and 189 spots showing the opposite pattern. Strikingly, 3 of 4 proteins consistently identified as different at a greater than 5-fold level between E. histolytica HM-1∶IMSS and E. dispar were identical to proteins recently identified as differentially expressed between E. histolytica HM-1∶IMSS and the reduced virulence strain E. histolytica Rahman. One of these was E. histolytica alcohol dehydrogenase 3 (EhADH3). We found that E. histolytica possesses a higher level of NADP-dependent alcohol dehydrogenase activity than E. dispar and that some EhADH3 can be localized to the surface of E. histolytica. Episomal overexpression of EhADH3 in E. histolytica trophozoites resulted in only subtle phenotypic differences in E. histolytica virulence in animal models of amebic colitis and amebic liver abscess, making it difficult to directly link EhADH3 levels to virulence differences between E. histolytica and less-pathogenic Entamoeba.
机译：原生动物肠内寄生虫组织型变形杆菌（Entamoeba histolytica）感染全球数百万人，并能够引起阿米巴痢疾和阿米巴肝脓肿。紧密相关的Entamoeba物种分散了更多的人，但是这种生物不会诱发疾病。为了确定这两种生物之间的分子差异，这可能是造成人类疾病的能力差异的原因，我们使用了基于二维凝胶（DIGE）的蛋白质组学分析方法来比较溶血性大肠杆菌和dispar的全细胞裂解液。我们观察到141个斑点在溶组织性大肠杆菌HM-1∶IMSS中的表达水平明显高于（大于5倍）dispar。E. dispar和189个斑点显示了相反的模式。引人注目的是，4种蛋白质中的3种被一致鉴定为在溶组织性大肠杆菌HM-1∶IMSS和大肠埃希氏菌之间差异大于5倍的水平上与最近鉴定为在组织溶性大肠杆菌HM-1∶IMSS和大肠埃希氏菌之间差异表达的蛋白相同。降低毒力的菌株E. histolytica Rahman。其中之一是组织溶解大肠杆菌乙醇脱氢酶3（EhADH3）。我们发现，溶组织性大肠杆菌具有比dispar。Epar更高的NADP依赖性酒精脱氢酶活性，并且一些EhADH3可以定位于溶组织性大肠杆菌的表面。在溶血性阿米巴性结肠炎和阿米巴性肝脓肿的动物模型中，溶组织性大肠杆菌营养体中EhADH3的过量表达仅导致溶组织性大肠杆菌致病性的细微表型差异，从而难以将EhADH3水平与 E之间的致病性差异直接联系起来。组织溶菌和病原性较低的 Entamoeba 。

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