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The Rhizosphere Bacterial Microbiota of Vitis vinifera cv. Pinot Noir in an Integrated Pest Management Vineyard

机译:葡萄(Vitis vinifera cv)的根际细菌菌群。黑比诺在虫害综合治理葡萄园中

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摘要

Microorganisms associated with Vitis vinifera (grapevine) can affect its growth, health and grape quality. The aim of this study was to unravel the biodiversity of the bacterial rhizosphere microbiota of grapevine in an integrated pest management vineyard located in Piedmont, Italy. Comparison between the microbial community structure in the bulk and rhizosphere soil (variable: space) were performed. Moreover, the possible shifts of the bulk and rhizosphere soil microbiota according to two phenological stages such as flowering and early fruit development (variable: time) were characterized. The grapevine microbiota was identified using metagenomics and next-generation sequencing. Biodiversity was higher in the rhizosphere than in the bulk soil, independent of the phenological stage. Actinobacteria were the dominant class with frequencies ≥ 50% in all the soil samples, followed by Proteobacteria, Gemmatimonadetes, and Bacteroidetes. While Actinobacteria and Proteobacteria are well-known as being dominant in soil, this is the first time the presence of Gemmatimonadetes has been observed in vineyard soils. Gaiella was the dominant genus of Actinobacteria in all the samples. Finally, the microbiota associated with grapevine differed from the bulk soil microbiota and these variations were independent of the phenological stage of the plant.
机译:与葡萄(葡萄)相关的微生物会影响其生长,健康和葡萄品质。这项研究的目的是在意大利皮埃蒙特的一个综合病虫害管理葡萄园中揭示葡萄的细菌根际微生物群的生物多样性。比较了块状土壤和根际土壤中的微生物群落结构(变量:空间)。此外,根据两个物候学阶段(如开花和果实早期发育(变量:时间)),对块状和根际土壤微生物区系的可能移动进行了表征。使用宏基因组学和下一代测序技术鉴定了葡萄菌群。与物候阶段无关,根际中的生物多样性高于散土中的生物多样性。放线菌是所有土壤样品中频率≥50%的主要类别,其次是变形杆菌,芽孢杆菌和拟杆菌。虽然众所周知放线菌和变形杆菌在土壤中占主导地位,但这是第一次在葡萄园土壤中发现芽孢杆菌属。盖氏菌是所有样品中放线菌的主要属。最后,与葡萄藤相关的微生物群不同于土壤微生物群,这些变化与植物的物候阶段无关。

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