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首页> 美国卫生研究院文献>other >Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process
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Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process

机译：山核桃（嫁接山核桃）嫁接工会的比较蛋白质组学分析提供了对嫁接过程的响应机制的见解。

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Hickory (Carya cathayensis), a tree with high nutritional and economic value, is widely cultivated in China. Grafting greatly reduces the juvenile phase length and makes the large scale cultivation of hickory possible. To reveal the response mechanisms of this species to grafting, we employed a proteomics-based approach to identify differentially expressed proteins in the graft unions during the grafting process. Our study identified 3723 proteins, of which 2518 were quantified. A total of 710 differentially expressed proteins (DEPs) were quantified and these were involved in various molecular functional and biological processes. Among these DEPs, 341 were up-regulated and 369 were down-regulated at 7 days after grafting compared with the control. Four auxin-related proteins were down-regulated, which was in agreement with the transcription levels of their encoding genes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the ‘Flavonoid biosynthesis’ pathway and ‘starch and sucrose metabolism’ were both significantly up-regulated. Interestingly, five flavonoid biosynthesis-related proteins, a flavanone 3-hyfroxylase, a cinnamate 4-hydroxylase, a dihydroflavonol-4-reductase, a chalcone synthase, and a chalcone isomerase, were significantly up-regulated. Further experiments verified a significant increase in the total flavonoid contents in scions, which suggests that graft union formation may activate flavonoid biosynthesis to increase the content of a series of downstream secondary metabolites. This comprehensive analysis provides fundamental information on the candidate proteins and secondary metabolism pathways involved in the grafting process for hickory.
机译：山核桃（山核桃），具有很高的营养和经济价值，在中国广泛种植。嫁接大大减少了幼年的相长，使山核桃的大规模种植成为可能。为了揭示该物种对嫁接的响应机制，我们采用了基于蛋白质组学的方法来在嫁接过程中鉴定出在嫁接接头中差异表达的蛋白质。我们的研究鉴定了3723种蛋白质，其中2518种被定量。总共对710个差异表达蛋白（DEP）进行了定量，这些蛋白参与了各种分子功能和生物学过程。与对照相比，在移植后7天，这些DEP中有341个上调，有369个下调。四种生长素相关蛋白被下调，这与其编码基因的转录水平一致。京都基因与基因组百科全书（KEGG）分析显示，“类黄酮生物合成”途径和“淀粉和蔗糖代谢”均显着上调。有趣的是，五个类黄酮生物合成相关的蛋白质，黄烷酮3-羟化酶，肉桂酸4-羟化酶，二氢黄酮醇4-还原酶，查尔酮合酶和查尔酮异构酶被显着上调。进一步的实验证实了接穗中总黄酮含量的显着增加，这表明嫁接结合的形成可能会激活黄酮生物合成，从而增加一系列下游次级代谢产物的含量。这种全面的分析提供了有关山核桃嫁接过程中候选蛋白质和次级代谢途径的基本信息。

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