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Response Surface Methodology for Optimizing the Production of Biosurfactant by Candida tropicalis on Industrial Waste Substrates

机译：响应面方法优化热带假丝酵母在工业废物基质上生产生物表面活性剂

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Biosurfactant production optimization by Candida tropicalis UCP0996 was studied combining central composite rotational design (CCRD) and response surface methodology (RSM). The factors selected for optimization of the culture conditions were sugarcane molasses, corn steep liquor, waste frying oil concentrations and inoculum size. The response variables were surface tension and biosurfactant yield. All factors studied were important within the ranges investigated. The two empirical forecast models developed through RSM were found to be adequate for describing biosurfactant production with regard to surface tension (R2 = 0.99833) and biosurfactant yield (R2 = 0.98927) and a very strong, negative, linear correlation was found between the two response variables studied (r = −0.95). The maximum reduction in surface tension and the highest biosurfactant yield were 29.98 mNm−1 and 4.19 gL−1, respectively, which were simultaneously obtained under the optimum conditions of 2.5% waste frying oil, 2.5%, corn steep liquor, 2.5% molasses, and 2% inoculum size. To validate the efficiency of the statistically optimized variables, biosurfactant production was also carried out in 2 and 50 L bioreactors, with yields of 5.87 and 7.36 gL−1, respectively. Finally, the biosurfactant was applied in motor oil dispersion, reaching up to 75% dispersion. Results demonstrated that the CCRD was suitable for identifying the optimum production conditions and that the new biosurfactant is a promising dispersant for application in the oil industry.
机译：结合中央复合旋转设计（CCRD）和响应面方法（RSM），研究了热带假丝酵母UCP0996对生物表面活性剂生产的优化。选择用于优化培养条件的因素是甘蔗糖蜜，玉米浆，废煎炸油的浓度和接种量。响应变量是表面张力和生物表面活性剂产量。在所研究的范围内，所有研究的因素都很重要。发现通过RSM建立的两个经验预测模型足以描述表面张力（R 2 = 0.99833）和生物表面活性剂产量（R 2 = 0.98927）方面的生物表面活性剂生产。 ），并且在所研究的两个响应变量之间发现非常强的负线性相关性（r = -0.95）。表面张力的最大降低和最高的生物表面活性剂产量分别为29.98 mNm -1 和4.19 gL -1 ，这是在2.5％废物的最佳条件下同时获得的。煎炸油，2.5％，玉米浆，2.5％糖蜜和2％接种量。为了验证统计优化变量的效率，还在2 L和50 L生物反应器中进行了生物表面活性剂的生产，产量分别为5.87和7.36 gL -1 。最后，将生物表面活性剂应用于机油分散液中，达到最高75％的分散度。结果表明，CCDR适用于确定最佳生产条件，而新型生物表面活性剂是在石油工业中应用的有希望的分散剂。

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