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首页> 美国卫生研究院文献>other >Whole Mitochondrial Genome Sequencing and Re-Examination of a Cytoplasmic Male Sterility-Associated Gene in Boro-Taichung-Type Cytoplasmic Male Sterile Rice
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Whole Mitochondrial Genome Sequencing and Re-Examination of a Cytoplasmic Male Sterility-Associated Gene in Boro-Taichung-Type Cytoplasmic Male Sterile Rice

机译：台中型细胞质雄性不育水稻中细胞质雄性不育相关基因的全线粒体基因组测序与复检

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摘要

Nuclear genome substitutions between subspecies can lead to cytoplasmic male sterility (CMS) through incompatibility between nuclear and mitochondrial genomes. Boro-Taichung (BT)-type CMS rice was obtained by substituting the nuclear genome of Oryza sativa subsp. indica cultivar Chinsurah Boro II with that of Oryza sativa subsp. japonica cultivar Taichung 65. In BT-type CMS rice, the mitochondrial gene orf79 is associated with male sterility. A complete sequence of the Boro-type mitochondrial genome responsible for BT-type CMS has not been determined to date. Here, we used pyrosequencing to construct the Boro-type mitochondrial genome. The contiguous sequences were assembled into five circular DNA molecules, four of which could be connected into a single circle. The two resulting subgenomic circles were unable to form a reliable master circle, as recombination between them was scarcely detected. We also found an unequal abundance of DNA molecules for the two loci of atp6. These results indicate the presence of multi-partite DNA molecules in the Boro-type mitochondrial genome. Expression patterns were investigated for Boro-type mitochondria-specific orfs, which were not found in the mitochondria from the standard japonica cultivar Nipponbare. Restorer of fertility 1 (RF1)-dependent RNA processing has been observed in orf79-containing RNA but was not detected in other Boro-type mitochondria-specific orfs, supporting the conclusion that orf79 is a unique CMS-associated gene in Boro-type mitochondria.
机译：亚种之间的核基因组取代可通过核基因组和线粒体基因组之间的不相容性导致细胞质雄性不育（CMS）。通过取代水稻（Oryza sativa）亚种的核基因组获得了Boro-Taichung（BT）型CMS水稻。 d稻Chinsurah Boro II和稻米亚种。粳稻台中65.在BT型CMS水稻中，线粒体基因orf79与雄性不育有关。迄今为止，尚未确定负责BT型CMS的Boro型线粒体基因组的完整序列。在这里，我们使用焦磷酸测序来构建Boro型线粒体基因组。连续序列被组装成五个环状DNA分子，其中四个可以连接成一个圆圈。由于几乎未检测到它们之间的重组，因此产生的两个亚基因组圈无法形成可靠的主控圈。我们还发现atp6的两个基因座的DNA分子丰度不均。这些结果表明在Boro型线粒体基因组中存在多部分DNA分子。研究了Boro型线粒体特异性orfs的表达模式，这在标准粳稻品种Nipponbare的线粒体中找不到。在包含orf79的RNA中观察到了受精1（RF1）依赖性RNA加工的恢复子，但在其他Boro型线粒体特异性orfs中未检测到，这支持了orf79是Boro型线粒体中独特的CMS相关基因的结论。 。

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    Tomohiko Kazama; Kinya Toriyama;

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  • 年(卷),期 -1(11),7
  • 年度 -1
  • 页码 e0159379
  • 总页数 13
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