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首页> 美国卫生研究院文献>other >Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase
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Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase

机译：亚细胞定位和复杂形成的蓝藻中氨酰基-tRNA合成酶：膜锚定的ValRS与ATP合酶相互作用的证据。

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摘要

tRNAs are charged with cognate amino acids by aminoacyl-tRNA synthetases (aaRSs) and subsequently delivered to the ribosome to be used as substrates for gene translation. Whether aminoacyl-tRNAs are channeled to the ribosome by transit within translational complexes that avoid their diffusion in the cytoplasm is a matter of intense investigation in organisms of the three domains of life. In the cyanobacterium Anabaena sp. PCC 7120, the valyl-tRNA synthetase (ValRS) is anchored to thylakoid membranes by means of the CAAD domain. We have investigated whether in this organism ValRS could act as a hub for the nucleation of a translational complex by attracting other aaRSs to the membranes. Out of the 20 aaRSs, only ValRS was found to localize in thylakoid membranes whereas the other enzymes occupied the soluble portion of the cytoplasm. To investigate the basis for this asymmetric distribution of aaRSs, a global search for proteins interacting with the 20 aaRSs was conducted. The interaction between ValRS and the FoF1 ATP synthase complex here reported is of utmost interest and suggests a functional link between elements of the gene translation and energy production machineries.
机译：tRNA通过氨酰基-tRNA合成酶（aaRS）带有同源氨基酸，随后被递送至核糖体，用作基因翻译的底物。氨酰基-tRNA是否通过翻译复合物中的转运而传递到核糖体，以避免它们在细胞质中扩散，这是对生命的三个领域的有机体的深入研究。在蓝藻鱼腥藻中。戊基-tRNA合成酶（ValRS）PCC 7120通过CAAD域固定在类囊体膜上。我们研究了在这种生物中，ValRS是否可以通过将其他aaRS吸引到膜上来充当翻译复合物成核的枢纽。在20个aaRS中，只有ValRS被发现位于类囊体膜中，而其他酶占据了细胞质的可溶性部分。为了研究这种aaRS不对称分布的基础，进行了与20个aaRS相互作用的蛋白质的整体搜索。在此报道的ValRS与FoF1 ATP合酶复合物之间的相互作用引起了极大的兴趣，并暗示了基因翻译元件与能量产生装置之间的功能联系。

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