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MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value

机译：来自辣木的MicroRNA：高通量测序鉴定及其对植物药用价值的潜在贡献

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摘要

Moringa oleifera is a widespread plant with substantial nutritional and medicinal value. We postulated that microRNAs (miRNAs), which are endogenous, noncoding small RNAs regulating gene expression at the post-transcriptional level, might contribute to the medicinal properties of plants of this species after ingestion into human body, regulating human gene expression. However, the knowledge is scarce about miRNA in Moringa. Furthermore, in order to test the hypothesis on the pharmacological potential properties of miRNA, we conducted a high-throughput sequencing analysis using the Illumina platform. A total of 31,290,964 raw reads were produced from a library of small RNA isolated from M. oleifera seeds. We identified 94 conserved and two novel miRNAs that were validated by qRT-PCR assays. Results from qRT-PCR trials conducted on the expression of 20 Moringa miRNA showed that are conserved across multiple plant species as determined by their detection in tissue of other common crop plants. In silico analyses predicted target genes for the conserved miRNA that in turn allowed to relate the miRNAs to the regulation of physiological processes. Some of the predicted plant miRNAs have functional homology to their mammalian counterparts and regulated human genes when they were transfected into cell lines. To our knowledge, this is the first report of discovering M. oleifera miRNAs based on high-throughput sequencing and bioinformatics analysis and we provided new insight into a potential cross-species control of human gene expression. The widespread cultivation and consumption of M. oleifera, for nutritional and medicinal purposes, brings humans into close contact with products and extracts of this plant species. The potential for miRNA transfer should be evaluated as one possible mechanism of action to account for beneficial properties of this valuable species.
机译：辣木是一种广泛的植物，具有重要的营养和药用价值。我们推测microRNA（miRNA）是内源的非编码小RNA，在转录后水平上调节基因表达，在摄入人体并调节人类基因表达后，可能有助于该种植物的药用特性。但是，关于辣木中的miRNA的知识很少。此外，为了检验关于miRNA的药理潜力的假设，我们使用Illumina平台进行了高通量测序分析。从油橄榄分枝杆菌种子分离的小RNA文库中产生了总计31,290,964个原始读段。我们鉴定了94个保守的miRNA和两个新颖的miRNA，这些均已通过qRT-PCR分析验证。通过qRT-PCR试验对20种辣木miRNA的表达进行的研究结果表明，通过在其他常见农作物植物组织中进行检测，可以确定多种植物物种之间的保守性。在计算机分析中，保守的miRNA的预测靶基因又将其与生理过程的调控联系起来。当将其转染到细胞系中时，某些预测的植物miRNA与它们的哺乳动物对应物具有功能同源性，并且可以调节人类基因。据我们所知，这是第一篇基于高通量测序和生物信息学分析发现油橄榄分枝杆菌miRNA的报告，我们提供了对人类基因表达的潜在跨物种控制的新见解。出于营养和药用目的，油橄榄的广泛种植和消费使人类与该植物物种的产品和提取物紧密接触。 miRNA转移的潜力应作为一种可能的作用机理进行评估，以解释该有价值物种的有益特性。

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