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Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: an innovative technique in food supplement quality control

机译:用于天然食品制备模式识别的植物化学指纹和化学计量学:食品添加剂质量控制中的创新技术

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摘要

Recently, the fingerprint approach using chromatography has become one of the most effective tools for quality assessment of herbal medicines and food supplements: due to the complexity of the chromatographic fingerprint and the irreproducibility of chromatographic instruments and experimental conditions, chemometric approach is employed to deal with the chromatographic fingerprint. The study was aimed at developing new analytical methods for the multivariate phytochemical fingerprinting of bioactive compounds in eight tree-species bud-preparations, commonly used in phytotherapy. Methods was used to identify and quantify the main bioactive compounds (polyphenols, organic acids and vitamins), and obtain a specific botanical profile in order to assess the contribution of each single bioactive class to the total bud preparation phytocomplex. A chemometric approach was used to distinguish among different genotypes assuring the identity, safety and quality of the botanical raw materials. The established protocol was simple, sensitive and reliable and it could be used for the evaluation and quality control of bud-extracts and natural food supplements: the proposed method was successfully applied to the characterization of commercial bud-preparations, demonstrating to be an effective tool for the fingerprinting of this plant material. The new approach developed in this study represents a good alternative for improving the classification results of herbal materials with complex chromatograms. It should be necessary to develop a “multivariate chromatographic fingerprint”, in order to differentiate the herbal preparations according to their genotype, avoiding substitutions, changes or adulterations with other species or synthetic drugs.Electronic supplementary materialThe online version of this article (doi:10.1007/s13197-015-2115-6) contains supplementary material, which is available to authorized users.
机译:近年来,使用色谱法的指纹方法已成为用于草药和食品补充剂质量评估的最有效工具之一:由于色谱指纹图谱的复杂性以及色谱仪器和实验条件的不可再现性,因此采用化学计量法来处理色谱指纹图谱。这项研究旨在开发新的分析方法,用于对八种常用于植物疗法的树种芽制剂中生物活性化合物进行多元植物化学指纹分析。方法用于鉴定和量化主要的生物活性化合物(多酚,有机酸和维生素),并获得特定的植物特征,以评估每种单一生物活性类别对总芽制备植物复合物的贡献。化学计量学方法用于区分不同的基因型,以确保植物原料的身份,安全性和质量。所建立的方案简单,灵敏,可靠,可用于芽提取物和天然食品补充剂的评估和质量控制:所提出的方法已成功地应用于商业芽制剂的表征,证明是一种有效的工具用于这种植物材料的指纹。这项研究中开发的新方法是改善具有复杂色谱图的草药材料分类结果的一个很好的选择。为了根据草药的基因型区分草药制剂,避免与其他物种或合成药物的替代,变化或掺假,有必要开发“多元色谱指纹图谱”。电子补充材料本文的在线版本(doi:10.1007) / s13197-015-2115-6)包含补充材料,授权用户可以使用。

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