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首页> 美国卫生研究院文献>Journal of Food Science and Technology >Chemical composition antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts
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Chemical composition antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts

机译：不同加工的灵芝乙醇提取物的化学成分抗增殖和抗氧化活性

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The content of phenolic compounds (TPC) and glucans, as well as the effectiveness of antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts were determined and compared. The content of glucans (total, α- and β-) strongly depended on the extraction time and particle size, but only interaction of these parameters influenced the TPC. Gallic acid, quercetin, trans-cinnamic acid, kaempferol, hesperetin and naringenin were detected in extracts by HPLC–DAD. The most abundant phenols were hesperetin (1.875–3.222 µg/g) and naringenin (1.235–2.856 µg/g). The ethanol extracts exhibited noteworthy antioxidant activity, but the significant amount of phenolic compounds was strongly linked to polysaccharides, and hence reduced their antioxidant capacity. The results of the antiproliferative activity in vitro showed that the analyzed extracts were the most effective against HeLa cells. Significant correlations were observed between the antiproliferative effect and the TPC/glucan content of extracts.
机译：测定并比较了酚类化合物（TPC）和葡聚糖的含量，以及不同加工的灵芝乙醇提取物的抗增殖和抗氧化活性。葡聚糖的含量（总α-和β-）在很大程度上取决于提取时间和粒径，但是只有这些参数的相互作用才影响TPC。 HPLC-DAD提取物中检出了没食子酸，槲皮素，反肉桂酸，山奈酚，橙皮素和柚皮素。最丰富的酚是橙皮素（1.875–3.222 µg / g）和柚皮素（1.235–2.856 µg / g）。乙醇提取物表现出显着的抗氧化活性，但是大量的酚类化合物与多糖紧密相连，因此降低了其抗氧化能力。体外抗增殖活性的结果表明，所分析的提取物对HeLa细胞最有效。观察到抗增殖作用与提取物的TPC /葡聚糖含量之间存在显着相关性。

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