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首页> 美国卫生研究院文献>Frontiers in Plant Science >Genome-Wide Linkage Mapping of QTL for Yield Components Plant Height and Yield-Related Physiological Traits in the Chinese Wheat Cross Zhou 8425B/Chinese Spring
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Genome-Wide Linkage Mapping of QTL for Yield Components Plant Height and Yield-Related Physiological Traits in the Chinese Wheat Cross Zhou 8425B/Chinese Spring

机译：中国小麦杂交周8425B /中国春QTL的全基因组连锁图谱及其产量构成株高和生理相关性状

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Identification of genes for yield components, plant height (PH), and yield-related physiological traits and tightly linked molecular markers is of great importance in marker-assisted selection (MAS) in wheat breeding. In the present study, 246 F8 RILs derived from the cross of Zhou 8425B/Chinese Spring were genotyped using the high-density Illumina iSelect 90K single nucleotide polymorphism (SNP) assay. Field trials were conducted at Zhengzhou and Zhoukou of Henan Province, during the 2012–2013 and 2013–2014 cropping season under irrigated conditions, providing data for four environments. Analysis of variance (ANOVA) of agronomic and physiological traits revealed significant differences (P < 0.01) among RILs, environments, and RILs × environments interactions. Broad-sense heritabilities of all traits including thousand kernel weight (TKW), PH, spike length (SL), kernel number per spike (KNS), spike number/m2 (SN), normalized difference in vegetation index at anthesis (NDVI-A) and at 10 days post-anthesis (NDVI-10), SPAD value of chlorophyll content at anthesis (Chl-A) and at 10 days post-anthesis (Chl-10) ranged between 0.65 and 0.94. A linkage map spanning 3609.4 cM was constructed using 5636 polymorphic SNP markers, with an average chromosome length of 171.9 cM and marker density of 0.64 cM/marker. A total of 866 SNP markers were newly mapped to the hexaploid wheat linkage map. Eighty-six QTL for yield components, PH, and yield-related physiological traits were detected on 18 chromosomes except 1D, 5D, and 6D, explaining 2.3–33.2% of the phenotypic variance. Ten stable QTL were identified across four environments, viz. QTKW.caas-6A.1, QTKW.caas-7AL, QKNS.caas-4AL, QSN.caas-1AL.1, QPH.caas-4BS.2, QPH.caas-4DS.1, QSL.caas-4AS, QSL.caas-4AL.1, QChl-A.caas-5AL, and QChl-10.caas-5BL. Meanwhile, 10 QTL-rich regions were found on chromosome 1BS, 2AL (2), 3AL, 4AL (2), 4BS, 4DS, 5BL, and 7AL exhibiting pleiotropic effects. These QTL or QTL clusters are tightly linked to SNP markers, with genetic distances to the closest SNPs ranging from 0 to 1.5 cM, and could serve as target regions for fine mapping, candidate gene discovery, and MAS in wheat breeding.
机译：鉴定小麦产量标记，植物高度（PH），与产量相关的生理性状和紧密相连的分子标记的基因在小麦育种的标记辅助选择（MAS）中非常重要。在本研究中，使用高密度Illumina iSelect 90K单核苷酸多态性（SNP）分析对来自周8425B /中国春季杂交的246个F8 RIL进行了基因分型。在灌溉条件下的2012–2013年和2013–2014年作物种植季节，在河南省郑州和周口进行了田间试验，提供了四种环境的数据。农艺和生理性状的方差分析（ANOVA）显示，RIL，环境和RIL×环境相互作用之间存在显着差异（P <0.01）。所有性状的广义遗传力，包括千粒重（TKW），PH，穗长（SL），每穗粒数（KNS），穗数/ m 2 （SN），归一化差异花期（NDVI-A）和花后10天（NDVI-10）的植被指数，花期（Chl-A）和花后10天（Chl-10）的叶绿素含量的SPAD值介于0.65和0.94。使用5636个多态性SNP标记构建了一个跨越3609.4 cM的连锁图，平均染色体长度为171.9 cM，标记密度为0.64 cM /标记。总共866个SNP标记被新定位到六倍体小麦连锁图谱上。在除1D，5D和6D之外的18条染色体上检测到86个QTL的产量成分，PH和与产量相关的生理特性，解释了2.3–33.2％的表型变异。在四个环境中确定了十个稳定的QTL，即。 QTKW.caas-6A.1，QTKW.caas-7AL，QKNS.caas-4AL，QSN.caas-1AL.1，QPH.caas-4BS.2，QPH.caas-4DS.1，QSL.caas-4AS， QSL.caas-4AL.1，QChl-A.caas-5AL和QChl-10.caas-5BL。同时，在染色体1BS，2AL（2），3AL，4AL（2），4BS，4DS，5BL和7AL染色体上发现了10个富含QTL的区域，表现出多效性。这些QTL或QTL簇与SNP标记紧密相连，与最接近的SNP的遗传距离范围为0至1.5 cM，可以用作小麦育种中精细定位，候选基因发现和MAS的目标区域。

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