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首页> 美国卫生研究院文献>other >Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris)
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Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris)

机译：犬肉单胞菌和Sarcocystis svanai n。 spp。 （Apicomplexa：Sarcocystidae）与家犬（犬似犬）中的严重肌炎和肝炎相关

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There are several reports of Sarcocystis sarcocysts in muscles of dogs but these species have not been named. Additionally, there are 2 reports of Sarcocystis neurona in dogs. Here, we propose 2 new names, Sarcocystis caninum, and Sarcocystis svanai for sarcocysts associated with clinical muscular sarcocystosis in 4 domestic dogs (Canis familiaris), 1 each from Montana and Colorado in the USA, and 2 from British Columbia, Canada. Only the sarcocyst stage was identified. Most of the sarcocysts identified were S. caninum. Sarcocysts were studied using light microscopy, transmission electron microscopy, and PCR. Based on collective results 2 new species, Sarcocystis caninum and Sarcocystis svanai were designated. Sarcocystis caninum and Sarcocystis svanai were structurally distinct. Sarcocystis caninum sarcocysts were up to 1.2 mm long and up to 75 μm wide. By light microscopy, the sarcocyst wall was relatively thin and smooth. By transmission electron microscopy (TEM), the sarcocyst wall “type 9”, 1–2 μm thick, and contained villar protrusions that lacked microtubules. Bradyzoites in sections were 7–9 μm long. Sarcocysts of S. svanai were few and were identified by TEM. Sarcocystis svanai sarcocysts were “type 1”, thin walled (< 0.5 μm), and the wall lacked villar protrusions but had tiny blebs that did not invaginate. DNA was extracted either from infected frozen muscle biopsies or formalin-fixed paraffin-embedded sections. Dogs were either singly infected with S. caninum or multiply co-infected with S. caninum and S. svanai (the result of a mixed infection) based on multi-locus DNA sequencing and morphology. BLASTn analysis established that the sarcocysts identified in these dogs were similar to, but not identical to S. canis or S. arctosi, parasites found to infect polar bears (Ursus maritimus) or brown bears (Ursus arctosi), respectively. However, the S. caninum sequence showed 100% identify over the 18S rRNA region sequenced to that of S. arctica, a parasite known to infect Arctic foxes (Vulpes lagopus).
机译：有几种关于犬肌肉中的肉囊藻类囊肿的报道，但这些物种尚未命名。此外，还有2例狗中神经节肉囊肿的报告。在这里，我们提出了2个新名称，Sarcocystis caninum和Sarcocystis svanai，用于与4只家犬（犬似犬）的临床肌肉结节病相关的囊肿，美国蒙大拿州和科罗拉多州各有1个，加拿大不列颠哥伦比亚省有2个。仅发现了囊肿阶段。鉴定出的大多数囊肿是犬链球菌。使用光学显微镜，透射电子显微镜和PCR研究了囊肿。根据总体结果，指定了2个新物种，即犬小囊藻和斯氏囊藻。犬肉单胞菌和Sarcocystis svanai在结构上截然不同。犬肉单胞菌的肉囊长至1.2 mm长，宽至75μm。通过光学显微镜检查，肌囊壁相对较薄且光滑。通过透射电子显微镜（TEM），囊壁为“ 9型”，厚1–2μm，并包含缺乏微管的Villar突起。截面中的缓殖子长7–9μm。 s.svanai的囊肿很少，并通过TEM鉴定。结节藻类结节囊是“ 1型”，薄壁（<0.5μm），壁上没有维拉尔突起，但有细小泡，没有内陷。从感染的冷冻肌肉活检组织或福尔马林固定石蜡包埋的切片中提取DNA。根据多位点DNA测序和形态学，狗要么被犬链球菌单株感染，要么被犬链球菌和s.svanai多重感染（混合感染的结果）。 BLASTn分析证实，在这些狗中鉴定出的囊肿与 S相似但不相同。 canis 或 S。 arctosi 寄生虫分别感染北极熊（ marusmus ）或棕熊（ arctosi ）。但是， S。 caninum 序列在与 S序列相同的18S rRNA区域显示出100％的鉴定。 arctica ，一种已知可感染北极狐（ Vulpes lagopus ）的寄生虫。

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