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Indocyanine Green-Loaded Photoacoustic Nanodroplets – Dual Contrast Nanoconstructs for Enhanced Photoacoustic and Ultrasound Imaging

机译:吲哚菁绿载光声纳米液滴–用于增强光声和超声成像的双重对比纳米结构

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摘要

Recently, perfluorocarbon (PFC) nanodroplets were introduced as contrast agents for imaging and image-guided therapy. For example, in sonography, high-intensity ultrasound pulses were used to phase-transition liquid perfluorocarbon to produce gas microbubbles. More recently, perfluorocarbon nanodroplets with encapsulated gold nanorods were used as dual ultrasound/photoacoustic contrast agents. To expedite clinical translation, we synthesized and characterized ICG-loaded perfluorocarbon nanodroplets, i.e. constructs comprising biocompatible, non-toxic and biologically safe materials. We then demonstrated enhanced photoacoustic contrast through optically triggered phase transition of PFC nanodroplets and ultrasound contrast from the resulting PFC bubbles. We assessed the quality enhancement of photoacoustic and ultrasound images through analysis of contrast and contrast-to-noise ratio. We further investigated the changes in image contrast due to increased ambient temperature. Our studies suggest that ICG-loaded perfluorocarbon nanodroplets may become a valuable tool for various imaging modalities, and have promising therapeutic applications.
机译:最近,全氟化碳(PFC)纳米液滴被引入作为造影剂,用于成像和图像引导疗法。例如,在超声检查中,使用高强度超声脉冲对液态全氟化碳进行相变以产生气体微气泡。最近,具有包封的金纳米棒的全氟化碳纳米液滴被用作双重超声/光声造影剂。为了加快临床翻译速度,我们合成并表征了负载有ICG的全氟化碳纳米滴,即包含生物相容性,无毒和生物安全材料的构建体。然后,我们证明了通过光学触发的PFC纳米液滴的相变和来自所得PFC气泡的超声对比度,可以增强光声对比度。我们通过分析对比和对比噪声比来评估光声和超声图像的质量增强。我们进一步研究了由于环境温度升高而导致的图像对比度变化。我们的研究表明,载有ICG的全氟化碳纳米滴可能成为各种成像方式的有价值的工具,并具有广阔的治疗应用前景。

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