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Overexpression of a Cotton Gene That Encodes a Putative Transcription Factor of AP2/EREBP Family in Arabidopsis Affects Growth and Development of Transgenic Plants

机译：拟南芥中编码AP2 / EREBP家族的假定转录因子的棉花基因的过表达影响转基因植物的生长和发育。

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In the study, a gene encoding a putative ethylene response factor of AP2/EREBP family was isolated from cotton (Gossypium hirsutum) and designated as GhERF12. Sequence alignment showed that GhERF12 protein contains a central AP2/ERF domain (58 amino acids) with two functional conserved amino acid residues (ala14 and asp19). Transactivation assay indicated that GhERF12 displayed strong transcription activation activity in yeast cells, suggesting that this protein may be a transcriptional activator in cotton. Quantitative RT-PCR analysis showed that GhERF12 expression in cotton was induced by ACC and IAA. Overexpression of GhERF12 in Arabidopsis affected seedling growth and development. The GhERF12 transgenic plants grew slowly, and displayed a dwarf phenotype. The mean bolting time of the transgenic plants was delayed for about 10 days, compared with that of wild type. Further study revealed that some ethylene-related and auxin-related genes were dramatically up-regulated in the transgenic plants, compared with those of wild type. Collectively, we speculated that GhERF12, as a transcription factor, may be involved in regulation of plant growth and development by activating the constitutive ethylene response likely related to auxin biosynthesis and/or signaling.

机译：在这项研究中，从棉花（陆地棉）中分离出一个编码假定的AP2 / EREBP乙烯反应因子的基因，并将其命名为GhERF12。序列比对显示，GhERF12蛋白包含一个中心AP2 / ERF结构域（58个氨基酸），带有两个功能保守的氨基酸残基（ala14和asp19）。反式激活分析表明，GhERF12在酵母细胞中表现出强大的转录激活活性，表明该蛋白可能是棉花中的转录激活因子。定量RT-PCR分析表明，棉花中的GhERF12表达受到ACC和IAA的诱导。 GhERF12在拟南芥中的过表达影响了幼苗的生长和发育。 GhERF12转基因植物生长缓慢，并表现出矮小的表型。与野生型相比，转基因植物的平均抽ing时间延迟了约10天。进一步的研究表明，与野生型植物相比，转基因植物中一些乙烯相关和植物生长素相关基因被显着上调。我们共同推测，GhERF12作为一种转录因子，可能通过激活可能与生长素生物合成和/或信号传导有关的组成型乙烯反应而参与植物生长和发育的调控。

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期刊名称 other
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Ying Zhou; Hui Xia; Xiao-Jie Li; Rong Hu; Yun Chen; Xue-Bao Li;
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年(卷),期 -1(8),10
年度 -1
页码 e78635
总页数 11
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专利

1. Over-expression of JcDREB, a putative AP2/EREBP domain-containing transcription factor gene in woody biodiesel plant Jatropha curcas, enhances salt and freezing tolerance in transgenic Arabidopsis thaliana. (Special Issue: Bioenergy plants in the United States and China.) [J] . Tang MingJuan, Liu XiaoFei, Deng HuaPing, Plant Science: An International Journal of Experimental Plant Biology . 2011,第6期

机译：木本生物柴油植物麻疯树中表达AP2 / EREBP域的转录因子基因 JcDREB 的过量表达增强了转基因拟南芥的耐盐和耐冻性。（特刊：美国和中国的生物能源工厂。）
2. An Annotation Update via cDNA Sequence Analysis and Comprehensive Profiling of Developmental, Hormonal or Environmental Responsiveness of the Arabidopsis AP2/EREBP Transcription Factor Gene Family [J] . Jian-Xun Feng, Di Liu, Yi Pan, Plant Molecular Biology . 2005,第6期

机译：通过拟南芥AP2 / EREBP转录因子基因家族的cDNA序列分析和发育，激素或环境响应综合分析进行注释更新。
3. An annotation update via cDNA sequence analysis and comprehensive profiling of developmental, hormonal or environmental responsiveness of the Arabidopsis AP2/EREBP transcription factor gene family [J] . Feng JX, Liu D, Pan Y, Plant Molecular Biology . 2005,第6期

机译：通过拟南芥AP2 / EREBP转录因子基因家族的cDNA序列分析和发育，激素或环境响应能力的综合分析进行注释更新
4. Isolation and characterization of novel transcription factors EREBPs containing conserved EREBP/AP2 domain in Triticum aestivum. [C] . Z. S. Xu, Y. Z. Ma, X. G. Cheng, International Wheat Genetics Symposium . 2003

机译：小鼠含有保守的eREBP / AP2结构域的新型转录因子Erebps的分离与表征。
5. Phylogenetic analysis of the AP2/ERF and KNOTTED1-like homeodomain families of transcription factors and finding of new members in Zea mais and Arabidopsis thaliana. [D] . Magnani, Enrico. 2007

机译：系统发育分析的AP2 / ERF和类似KNOTTED1同源域的转录因子家族，并在玉米和拟南芥中发现新成员。
6. Overexpression of the Tobacco Tsi1 Gene Encoding an EREBP/AP2–Type Transcription Factor Enhances Resistance against Pathogen Attack and Osmotic Stress in Tobacco [O] . Jeong Mee Park, Chang-Jin Park, Suk-Bae Lee, 2001

机译：编码EREBP / AP2型转录因子的烟草Tsi1基因的过表达增强了烟草对病原体侵害和渗透胁迫的抵抗力
7. Overexpression of a cotton gene that encodes a putative transcription factor of AP2/EREBP family in Arabidopsis affects growth and development of transgenic plants. [O] . Ying Zhou, Hui Xia, Xiao-Jie Li, 2013

机译：编码拟南芥中ap2 / EREBp家族的推定转录因子的棉花基因的过表达影响转基因植物的生长和发育。

Overexpression of a Cotton Gene That Encodes a Putative Transcription Factor of AP2/EREBP Family in Arabidopsis Affects Growth and Development of Transgenic Plants

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