Nanobubbles (<200 nm in diameter) have several unique properties such as long lifetime in liquid owing to its negatively charged surface, and its high gas solubility into the liquid owing to its high internal pressure. They are used in variety of fields including diagnostic aids and drug delivery, while there are no reports assessing their effects on the growth of lives. Nanobubbles of air or oxygen gas were generated using a nanobubble aerator (BUVITAS; Ligaric Company Limited, Osaka, Japan). Brassica campestris were cultured hydroponically for 4 weeks within air-nanobubble water or within normal water. Sweetfish (for 3 weeks) and rainbow trout (for 6 weeks) were kept either within air-nanobubble water or within normal water. Finally, 5 week-old male DBA1/J mice were bred with normal free-chaw and free-drinking either of oxygen-nanobubble water or of normal water for 12 weeks. Oxygen-nanobubble significantly increased the dissolved oxygen concentration of water as well as concentration/size of nanobubbles which were relatively stable for 70 days. Air-nanobubble water significantly promoted the height (19.1 vs. 16.7 cm; P<0.05), length of leaves (24.4 vs. 22.4 cm; P<0.01), and aerial fresh weight (27.3 vs. 20.3 g; P<0.01) of Brassica campestris compared to normal water. Total weight of sweetfish increased from 3.0 to 6.4 kg in normal water, whereas it increased from 3.0 to 10.2 kg in air-nanobubble water. In addition, total weight of rainbow trout increased from 50.0 to 129.5 kg in normal water, whereas it increased from 50.0 to 148.0 kg in air-nanobubble water. Free oral intake of oxygen-nanobubble water significantly promoted the weight (23.5 vs. 21.8 g; P<0.01) and the length (17.0 vs. 16.1 cm; P<0.001) of mice compared to that of normal water. We have demonstrated for the first time that oxygen and air-nanobubble water may be potentially effective tools for the growth of lives.
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机译：纳米气泡（直径小于200 nm）具有几个独特的特性，例如由于其带负电荷的表面而在液体中的使用寿命长，以及由于其内部压力高而在液体中的高气体溶解度。它们被用于各种领域，包括诊断辅助工具和药物输送，但没有报告评估它们对生命增长的影响。使用纳米气泡曝气器（BUVITAS； Ligaric Company Limited，日本大阪）产生空气或氧气的纳米气泡。将油菜在空气纳米水或普通水中水培培养4周。将甜鱼（3周）和虹鳟鱼（6周）放在空气纳米气泡水中或正常水中。最后，将5周大的雄性DBA1 / J小鼠与正常的自由咀嚼和氧气-纳米气泡水或正常的水自由饮水饲养12周。氧纳米气泡显着增加了水中溶解氧的浓度以及相对稳定70天的纳米气泡的浓度/大小。空气纳米气泡水显着提高了高度（19.1 vs. 16.7 cm； P <0.05），叶片长度（24.4 vs. 22.4 cm； P <0.01）和空气新鲜重量（27.3 vs. 20.3 g； P <0.01）。与普通水相比在正常水中，甜鱼的总重量从3.0千克增加到6.4千克，而在空气纳米气泡水中的总重量从3.0千克增加到10.2千克。此外，普通水中虹鳟鱼的总重量从50.0千克增加到129.5千克，而空气纳米气泡水中的虹鳟的总重量从50.0千克增加到148.0千克。与正常水相比，自由口服氧气-纳米气泡水显着提高了小鼠的体重（23.5对21.8 g； P <0.01）和长度（17.0对16.1 cm； P <0.001）。我们首次证明氧气和空气中的纳米气泡水可能是生命增长的潜在有效工具。
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