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Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes

机译：针对基因表达到转基因AEDES AEGYPTI蚊子的雌性幼虫脂肪体

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摘要

As the fat body is a critical tissue for mosquito development, metamorphosis, immune and reproductive system function, characterization of regulatory modules targeting gene expression to the female mosquito fat body at distinct life stages is much needed for multiple, varied strategies for controlling vector-borne diseases such as dengue and malaria. The hexameric storage protein, Hexamerin-1.2, of the mosquito, Aedes atropalpus, is female-specific and uniquely expressed in the fat body of fourth-instar larvae and young adults. We have identified in the Hex-1.2 gene, a short regulatory module that directs female-, tissue-, and stage-specific lacZ reporter gene expression using a heterologous promoter in transgenic lines of the dengue vector, Aedes aegypti. Male transgenic larvae and pupae of one line expressed no E. coli β-galactosidase or transgene product; in two other lines reporter gene activity was highly female-biased. All transgenic lines expressed the reporter only in the fat body. However, lacZ mRNA levels were no different in males and females at all stages examined, suggesting that the gene regulatory module drives female-specific expression by post-transcriptional regulation in the heterologous mosquito. This regulatory element from the Hex-1.2 gene thus provides a new molecular tool for transgenic mosquito control as well as functional genetic analysis in aedine mosquitoes.
机译：由于脂肪体是蚊子发育，变态，免疫和生殖系统功能的关键组织，因此需要多种多样的控制媒介传播策略的方法来表征在不同生命阶段针对女性蚊子脂肪体的基因表达的调控模块登革热和疟疾等疾病。蚊子伊蚊（Aedes atropalpus）的六聚体贮藏蛋白Hexamerin-1.2是女性特有的，并且在四龄幼虫和年轻人的脂肪体内独特表达。我们已经在Hex-1.2基因中发现了一个短的调控模块，该模块在登革热载体埃及伊蚊的转基因系中使用异源启动子指导女性，组织和阶段特定的lacZ报告基因表达。一系的雄性转基因幼虫和p未表达大肠杆菌β-半乳糖苷酶或转基因产物。在另外两行中，报告基因的活性高度偏向女性。所有转基因品系仅在脂肪体内表达报道基因。但是，lacZ mRNA水平在所有检查的雄性和雌性中均无差异，这表明基因调控模块通过异源蚊子的转录后调控来驱动雌性特异性表达。因此，来自Hex-1.2基因的调控元件为转基因蚊子的控制以及伊甸蚊的功能遗传分析提供了一种新的分子工具。

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