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首页> 外文期刊>Insect Molecular Biology >Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes.
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Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes.

机译：将基因表达靶向转基因埃及伊蚊的雌性幼虫脂肪体。

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As the fat body is a critical tissue for mosquito development, metamorphosis, immune and reproductive system function, the characterization of regulatory modules targeting gene expression to the female mosquito fat body at distinct life stages is much needed for multiple, varied strategies for controlling vector-borne diseases such as dengue and malaria. The hexameric storage protein, Hexamerin-1.2, of the mosquito Aedes atropalpus is female-specific and uniquely expressed in the fat body of fourth instar larvae and young adults. We have identified in the Hex-1.2 gene, a short regulatory module that directs female-, tissue-, and stage-specific lacZ reporter gene expression using a heterologous promoter in transgenic lines of the dengue vector Aedes aegypti. Male transgenic larvae and pupae of one line expressed no Escherichia coli beta -galactosidase or transgene product; in two other lines reporter gene activity was highly female-biased. All transgenic lines expressed the reporter only in the fat body; however, lacZ mRNA levels were no different in males and females at any stage examined, suggesting that the gene regulatory module drives female-specific expression by post-transcriptional regulation in the heterologous mosquito. This regulatory element from the Hex-1.2 gene thus provides a new molecular tool for transgenic mosquito control as well as functional genetic analysis in aedine mosquitoes.Digital Object Identifier http://dx.doi.org/10.1111/imb.12005
机译：由于脂肪体是蚊子发育，变态，免疫和生殖系统功能的关键组织，因此需要多种多样的策略来控制蚊子在不同生命阶段针对雌性蚊子脂肪体的基因表达调控模块的特征。传播疾病，例如登革热和疟疾。蚊伊蚊的六聚体存储蛋白Hexamerin-1.2是女性特有的，并且在第四龄幼虫和年轻成年人的脂肪体内独特表达。我们已经在Hex-1.2基因中发现了一个短的调控模块，该模块在登革热载体埃及伊蚊的转基因系中使用异源启动子指导女性，组织和阶段特定的lacZ报告基因表达。一系的雄性转基因幼虫和p未表达大肠杆菌β-半乳糖苷酶或转基因产物。在另外两行中，报道基因的活性高度偏向女性。所有转基因品系仅在脂肪体内表达了报道基因。但是，lacZ mRNA水平在任何检查阶段的雄性和雌性中均无差异，这表明基因调控模块通过异源蚊子的转录后调控来驱动雌性特异性表达。因此，来自Hex-1.2基因的这种调控元件为转基因蚊子的控制以及伊甸蚊的功能基因分析提供了一种新的分子工具。数字对象标识符http://dx.doi.org/10.1111/imb.12005

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