• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >MUC4 and MUC1 Expression in Adenocarcinoma of the Stomach Correlates with Vessel Invasion and Lymph Node Metastasis: An Immunohistochemical Study of Early Gastric Cancer
【2h】

MUC4 and MUC1 Expression in Adenocarcinoma of the Stomach Correlates with Vessel Invasion and Lymph Node Metastasis: An Immunohistochemical Study of Early Gastric Cancer

机译：mUC4和mUC1表达腺癌管浸润和淋巴结转移胃相关者：早期胃癌的免疫组化研究

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have previously reported that MUC4 expression is a poor prognostic factor in various carcinomas. Our previous study also showed that MUC1 expression in gastric cancers, including the early and advanced stages is a poor prognostic factor. In the present study, the expression profiles of MUC4 and MUC1 were examined by immunohistochemistry (IHC) using two anti-MUC4 monoclonal antibodies (MAbs), 8G7 and 1G8, and anti-MUC1 MAb DF3 in 104 gastrectomy specimens of early gastric adenocarcinoma with submucosal invasion (pT1b2), including 197 histological subtype lesions. Before the IHC study of the human specimens, we evaluated the specificity of the two MAbs by Western blotting and IHC of two MUC4 mRNA expressing gastric cancer cell lines. MAb 8G7 reacted clearly, whereas MAb 1G8 did not show any reactivity, in either Western blotting or IHC. In the IHC of the gastric cancers, the expression rates of MUC4/8G7 detected by MAb 8G7, MUC4/1G8 detected by MAb 1G8 and MUC1/DF3 detected by MAb DF3 in well differentiated types (70%, 38/54; 67%, 36/54; 52%, 28/54) were significantly higher than those in poorly differentiated types (18%, 10/55; 36%, 20/55; 13%, 7/55) (P<0.0001; P = 0.0021; P<0.0001), respectively. The MUC4/8G7 expression was related with lymphatic invasion (r = 0.304, P = 0.033). On the other hand, the MUC4/1G8 expression was related with lymphatic invasion (r = 0.395, P = 0.001) and lymph node metastasis (r = 0.296, P = 0.045). The MUC1/DF3 expression was related with lymphatic invasion (r = 0.357, P = 0.032) and venous invasion (r = 0.377, P = 0.024). In conclusion, the expression of MUC4 as well as MUC1 in early gastric cancers is a useful marker to predict poor prognostic factors related with vessel invasion.
机译：我们以前曾报道过，MUC4表达在各种癌症中是不良的预后因素。我们之前的研究还表明，MUC1在胃癌中的表达（包括早期和晚期）是不良的预后因素。在本研究中，使用两种抗MUC4单克隆抗体（MAb）8G7和1G8和抗MUC1 MAb DF3，通过免疫组织化学（IHC）在104例早期胃黏膜下腺癌胃切除术标本中检测了MUC4和MUC1的表达谱。侵袭（pT1b2），包括197个组织学亚型病变。在对人体标本进行IHC研究之前，我们通过蛋白质印迹和两种表达MUC4 mRNA的胃癌细胞系的IHC评估了两种单克隆抗体的特异性。 MAb 8G7反应清楚，而MAb 1G8在Western印迹或IHC中均未显示任何反应性。在高分化类型的胃癌IHC中，MAb 8G7检测到的MUC4 / 8G7，MAb 1G8检测到的MUC4 / 1G8和MAb DF3检测到的MUC1 / DF3在高分化类型中的表达率（70％，38/54; 67％， 36/54; 52％，28/54）显着高于低分化类型（18％，10/55; 36％，20/55; 13％，7/55）（P <0.0001; P = 0.0021 ； P <0.0001）。 MUC4 / 8G7的表达与淋巴管浸润有关（r = 0.304，P = 0.033）。另一方面，MUC4 / 1G8的表达与淋巴管浸润（r ＝ 0.395，P ＝ 0.001）和淋巴结转移（r ＝ 0.296，P ＝ 0.045）有关。 MUC1 / DF3的表达与淋巴管浸润（r = 0.357，P = 0.032）和静脉浸润（r = 0.377，P = 0.024）有关。总之，MUC4和MUC1在早期胃癌中的表达是预测与血管浸润相关的不良预后因素的有用标志。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号