• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure
【2h】

A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure

机译：绿色荧光蛋白含有一个QFG三肽发色团：光学特性和X射线晶体结构

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rtms5 is an deep blue weakly fluorescent GFP-like protein (, 592 nm; , 630nm; ΦF, 0.004) that contains a 66Gln-Tyr-Gly chromophore tripeptide sequence. We investigated the optical properties and structure of two variants, Rtms5Y67F and Rtms5Y67F/H146S in which the tyrosine at position 67 was substituted by a phenylalanine. Compared to the parent proteins the optical spectra for these new variants were significantly blue-shifted. Rtms5Y67F spectra were characterised by two absorbing species (, 440 nm and 513 nm) and green fluorescence emission (, 440 nm; , 508 nm; ΦF, 0.11), whilst Rtms5Y67F/H146S spectra were characterised by a single absorbing species (, 440 nm) and a relatively high fluorescence quantum yield (ΦF, 0.75; , 440 nm; , 508 nm). The fluorescence emissions of each variant were remarkably stable over a wide range of pH (3–11). These are the first GFP-like proteins with green emissions (500–520 nm) that do not have a tyrosine at position 67. The X-ray crystal structure of each protein was determined to 2.2 Å resolution and showed that the benzylidine ring of the chromophore, similar to the 4-hydroxybenzylidine ring of the Rtms5 parent, is non-coplanar and in the trans conformation. The results of chemical quantum calculations together with the structural data suggested that the 513 nm absorbing species in Rtms5Y67F results from an unusual form of the chromophore protonated at the acylimine oxygen. These are the first X-ray crystal structures for fluorescent proteins with a functional chromophore containing a phenylalanine at position 67.
机译：Rtms5是一种深蓝色的弱荧光GFP样蛋白（592 nm； 630 nm；ΦF，0.004），其中包含 66 Gln-Tyr-Gly发色团三肽序列。我们研究了两个变体Rtms5 Y67F 和Rtms5 Y67F / H146S 的光学性质和结构，其中67位的酪氨酸被苯丙氨酸取代。与亲本蛋白质相比，这些新变体的光谱发生了明显的蓝移。 Rtms5 Y67F 光谱的特征在于两种吸收物质（440 nm和513 nm）和绿色荧光发射（440 nm； 508 nm；ΦF，0.11），而Rtms5 Y67F / H146S 光谱的特征在于单一的吸收物种（440 nm）和相对较高的荧光量子产率（ΦF，0.75;，440 nm;，508 nm）。每个变体的荧光发射在很宽的pH范围（3-11）内都非常稳定。这些是第一个绿色发射（500-520 nm）的GFP样蛋白，在第67位没有酪氨酸。每种蛋白的X射线晶体结构确定为2.2Å的分辨率，表明该蛋白的苄基环发色团与Rtms5亲本的4-羟基苄基环相似，是非共面的，处于反式构象。化学量子计算的结果以及结构数据表明，Rtms5 Y67F 中的513 nm吸收物种是由在酰基亚胺氧上质子化的不寻常形式的发色团引起的。这些是荧光蛋白的第一个X射线晶体结构，其功能发色团在67位含有苯丙氨酸。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号