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首页> 外文会议>Molecular Imaging; Progress in Biomedical Optics and Imaging; vol.8 no.39; Proceedings of SPIE-The International Society for Optical Engineering; vol.6626 >Optical properties of green fluorescent proteins and their applications on virus infection
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Optical properties of green fluorescent proteins and their applications on virus infection

机译：绿色荧光蛋白的光学性质及其在病毒感染中的应用

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摘要

Exogenous fluorescent agents such as green fluorescent protein (GFP) have been widely used as biological indicators in bioimaging techniques. Although GFP and its mutants have been used in many applications, their optical properties have not been completely investigated, especially when they are under various environmental conditions. In this research, we developed a spectrum-analyzing system to investigate the fluorescent properties of GFP in the environments of different temperatures. We found that the fluorescent spectrum of GFP consisted of two components that might come from the transitions between different electronic energy states where the quantum efficiencies of the two components varied with different temperature. This effect was expected to come from the thermal effect on the electron populations in the molecular energy states of GFP. Furthermore, GFP was used as fluorescent marker to monitor the infection process of cells by viruses with a dynamic spectral imaging system. The recombinant baculoviruses containing the red and green fluorescent protein gene that can simultaneously produce dual fluorescence were used as vectors in insect cells. The system was used to monitor the spatial distribution of fluorescent spectra of cells infected by virus during the process of infection.
机译：外源荧光剂，例如绿色荧光蛋白（GFP），已被广泛用作生物成像技术中的生物指示剂。尽管GFP及其突变体已在许多应用中使用，但尚未完全研究其光学性质，尤其是当它们处于各种环境条件下时。在这项研究中，我们开发了一种光谱分析系统来研究GFP在不同温度环境下的荧光特性。我们发现GFP的荧光光谱由两个组分组成，这两个组分可能来自不同电子能态之间的跃迁，其中两个组分的量子效率随温度而变化。预期这种效应来自对GFP分子能态的电子种群的热效应。此外，GFP被用作荧光标记，通过动态光谱成像系统监测病毒对细胞的感染过程。含有能够同时产生双重荧光的红色和绿色荧光蛋白基因的重组杆状病毒被用作昆虫细胞中的载体。该系统用于监测在感染过程中被病毒感染的细胞的荧光光谱的空间分布。

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