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Use of green fluorescent protein to trace the infection of baculovirus in insects and to increase viral UV stability

机译:使用绿色荧光蛋白追踪昆虫中杆状病毒的感染并增加病毒的紫外线稳定性

摘要

Green fluorescent protein (GFP) gene was transplanted into baculovirus. The baculovirus was used to infect pests of vegetable crops. Three to five days after infection, the infected insects were found to emit strong fluorescence when exposed to a portable UV light. Therefore the spreading of baculovirus infection and disease progression can be effectively detected in the field. In addition, the GFP reduced UV damage to the baculovirus. As a result, GFP can increase the retention time of the virus as a pesticide in the field.
机译:将绿色荧光蛋白(GFP)基因移植到杆状病毒中。杆状病毒用于感染蔬菜作物的害虫。感染后三到五天,发现被感染的昆虫暴露于便携式紫外线下会发出强烈的荧光。因此,可以在现场有效地检测杆状病毒感染的传播和疾病的进展。另外,GFP减少了对杆状病毒的紫外线损害。结果,GFP可以增加该病毒在田间作为农药的保留时间。

著录项

  • 公开/公告号US5919445A

    专利类型

  • 公开/公告日1999-07-06

    原文格式PDF

  • 申请/专利权人 COUNCIL OF AGRICULTURE EXECUTIVE YUAN;

    申请/专利号US19970877564

  • 发明设计人 YU-CHAN CHAO;

    申请日1997-06-17

  • 分类号A01N63/00;C12N15/00;

  • 国家 US

  • 入库时间 2022-08-22 02:07:55

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