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Use of green fluorescent protein to trace the infection of baculovirus in insects and to increase viral UV stability
Use of green fluorescent protein to trace the infection of baculovirus in insects and to increase viral UV stability
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机译:使用绿色荧光蛋白追踪昆虫中杆状病毒的感染并增加病毒的紫外线稳定性
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摘要
Green fluorescent protein (GFP) gene was transplanted into baculovirus. The baculovirus was used to infect pests of vegetable crops. Three to five days after infection, the infected insects were found to emit strong fluorescence when exposed to a portable UV light. Therefore the spreading of baculovirus infection and disease progression can be effectively detected in the field. In addition, the GFP reduced UV damage to the baculovirus. As a result, GFP can increase the retention time of the virus as a pesticide in the field.
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