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OsLEA3-2 an Abiotic Stress Induced Gene of Rice Plays a Key Role in Salt and Drought Tolerance

机译:OsLEa3-2赖斯剧中的盐和耐旱性中起关键作用的非生物胁迫诱导基因

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摘要

Late embryogenesis abundant (LEA) proteins are involved in tolerance to drought, cold and high salinity in many different organisms. In this report, a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa) using the Rapid Amplification of cDNA Ends (RACE) method. OsLEA3-2 was found to be only expressed in the embryo and can be induced by abiotic stresses. The coding protein localizes to the nucleus and overexpression of OsLEA3-2 in yeast improved growth performance compared with control under salt- and osmotic-stress conditions. OsLEA3-2 was also inserted into pHB vector and overexpressed in Arabidopsis and rice. The transgenic Arabidopsis seedlings showed better growth on MS media supplemented with 150 mM mannitol or 100 mM NaCl as compared with wild type plants. The transgenic rice also showed significantly stronger growth performance than control under salinity or osmotic stress conditions and were able to recover after 20 days of drought stress. In vitro analysis showed that OsLEA3-2 was able to protect LDH from aggregation on freezing and inactivation on desiccation. These results indicated that OsLEA3-2 plays an important role in tolerance to abiotic stresses.
机译:晚期胚胎发生丰富(LEA)蛋白参与了许多不同生物体对干旱,寒冷和高盐分的耐受性。在此报告中,使用cDNA末端快速扩增(RACE)方法在水稻(Oryza sativa)中鉴定了产生LEA蛋白的全长基因OsLEA3-2。发现OsLEA3-2仅在胚胎中表达,并且可以由非生物胁迫诱导。与盐和渗透胁迫条件下的对照相比,编码蛋白位于酵母的核中,并且OsLEA3-2的过表达改善了生长性能。 OsLEA3-2也被插入pHB载体中,并在拟南芥和水稻中过表达。与野生型植物相比,转基因拟南芥幼苗在补充有150 mM甘露醇或100 mM NaCl的MS培养基上表现出更好的生长。在盐度或渗透胁迫条件下,转基因水稻的生长性能也明显强于对照,并且在干旱胁迫20天后能够恢复。体外分析表明,OsLEA3-2能够保护LDH免受冷冻时的聚集和干燥时的失活的影响。这些结果表明,OsLEA3-2在耐受非生物胁迫中起重要作用。

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    Jianli Duan; Weiming Cai;

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  • 年(卷),期 -1(7),9
  • 年度 -1
  • 页码 e45117
  • 总页数 11
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