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首页> 外文期刊>Journal of Biotechnology >Abiotic stress and ABA-inducible Group 4 LEA from Brassica napus plays a key role in salt and drought tolerance
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Abiotic stress and ABA-inducible Group 4 LEA from Brassica napus plays a key role in salt and drought tolerance

机译：甘蓝型油菜的非生物胁迫和ABA诱导的第4组LEA在耐盐和干旱中起关键作用

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Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2 and 3 LEAs among the nine different groups. The present study evaluates the functional role of a Group 4 LEA protein, LEA4-1 from Brassica napus. Expression analysis revealed that abscisic acid, salt, cold and osmotic stresses induce expression of LEA4-1 gene in leaf tissues in Brassica species. Conversely, reproductive tissues such as flowers and developing seeds showed constitutive expression of LEA4, which was up-regulated in flowers under salt stress. For functional evaluation of LEA4-1 with regard to stress tolerance, LEA4-1 cDNA was cloned from B. napus, and overexpressed in both Escherichia coli and transgenic Arabidopsis plants. Overexpression of BnLEA4-1 cDNA in E. coli conferred salt and extreme temperature tolerance to the transformed cells. Furthermore, transgenic Arabidopsis plants overexpressing BnLEA4-1 either under constitutive CaMV35S or abiotic stress inducible RD29A promoter showed enhanced tolerance to salt anddrought stresses. These results demonstrate that LEA4-1 plays a crucial role in abiotic stress tolerance during vegetative stage of plant development.
机译：胚后期富集（LEA）蛋白是亲水蛋白家族，形成种子的脱水耐受性不可或缺的一部分。 LEA蛋白也被假定在不同的非生物胁迫下起保护作用。在第9、2组中的第1、2和3组LEA中，它们在非生物胁迫耐受性中的作用已得到充分证明。本研究评估了来自甘蓝型油菜的第4组LEA蛋白LEA4-1的功能作用。表达分析表明，脱落酸，盐，冷和渗透胁迫均可诱导甘蓝型油菜叶片组织中LEA4-1基因的表达。相反，生殖组织如花和发育中的种子显示出LEA4的组成型表达，其在盐胁迫下在花中被上调。为了针对胁迫耐受性对LEA4-1进行功能评估，从甘蓝型油菜克隆了LEA4-1 cDNA，并在大肠杆菌和转基因拟南芥植物中均过表达。 BnLEA4-1 cDNA在大肠杆菌中的过表达赋予了盐和对转化细胞极高的温度耐受性。此外，在组成型CaMV35S或非生物胁迫诱导的RD29A启动子下过表达BnLEA4-1的转基因拟南芥植物对盐和干旱胁迫的耐受性增强。这些结果表明，LEA4-1在植物发育的营养阶段在非生物胁迫耐受性中起着至关重要的作用。

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