• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx
【2h】

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx

机译：测序和从头使用454从8个不同的发展阶段牡蛎角线分析（福建牡蛎）GsFLX

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Research on the mechanism for early development of shellfish, such as body plan, shell formation, settlement and metamorphosis is currently an active research field. However, studies were still limited and not deep enough because of the lack of genomic resources such as genome or transcriptome sequences. In the present research, de novo transcriptome sequencing was performed for Crassostrea angulata, the most economically important cultured oyster species in China, at eight early developmental stages using the 454 sequencing technology. A total of 555,215 reads were produced with an average length of 309 nucleotides that were then assembled into 10,462 contigs. As determined by GO annotation and KEGG pathway mapping, functional annotation of the unigenes recovered diverse biological functions and processes. Six unique sequences related to settlement, metamorphosis and growth were subsequently analyzed by real-time PCR. Given the lack of whole genome information for oysters, transcriptome and de novo analysis of C. angulata from the eight different developing phases will provide important and useful information on early development mechanism and help genetic breeding of shellfish.
机译：对贝类早期发育的机制，如身体计划，壳形成，沉降和变态的研究，目前是一个活跃的研究领域。但是，由于缺乏基因组资源（如基因组或转录组序列），研究仍然有限且不够深入。在本研究中，使用454测序技术在八个发展阶段对中国最经济的养殖牡蛎Crassostrea angulata进行了从头转录组测序。产生了总共555,215个读段，平均长度为309个核苷酸，然后将其组装成10,462个重叠群。通过GO注释和KEGG通路图谱确定，单基因的功能注释恢复了多种生物学功能和过程。随后通过实时PCR分析了与沉降，变态和生长相关的六个独特序列。由于缺乏牡蛎的全基因组信息，因此对八个不同发育阶段的Ang。C. angulata进行转录组分析和从头分析，将为早期发育机理提供重要而有用的信息，并有助于贝类的遗传育种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号