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Connexin 57 Is Expressed by the Axon Terminal Network of B-type Horizontal Cells in the Rabbit Retina

机译:连接蛋白57通过B型水平细胞的轴突终端网络在兔视网膜细胞表达

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摘要

In the rabbit retina there are two types of horizontal cell (HC). A-type HCs (AHC) are axonless and extensively coupled via connexin (Cx)50 gap junctions. The B-type HC (BHC) is axon-bearing; the somatic dendrites form a second network coupled by gap junctions while the axon terminals (ATs) form a third independent network in the outer plexiform layer (OPL). The mouse retina has only one type of HC, which is morphologically similar to the B-type HC of the rabbit. Previous work suggested that mouse HCs express Cx57 ( Eur J Neurosci 19:2633–2640). Therefore, we cloned rabbit Cx57 and raised an antibody to determine the distribution of Cx57 gap junctions among rabbit HCs. Dye injection methods were used to obtain detailed fills for all three HC networks for analysis by confocal microscopy. We found that Cx57 was associated with the B-type AT plexus. Cx57 plaques were anticorrelated with the B-type somatic dendrites and the A-type HC network. Furthermore, there was no colocalization between Cx50 and Cx57. We conclude that in the rabbit retina, Cx57 is only found on BHC-AT processes. Thus, in species where there are two types of HC, different connexins are expressed. The absence of Cx57 labeling in the somatic dendrites of B-type HCs suggests the possibility of an additional unidentified HC connexin in the rabbit.
机译:在兔视网膜中,有两种类型的水平细胞(HC)。 A型HCS(AHC)是通过Connexin(CX)50间隙结的轴突和广泛的耦合。 B型HC(BHC)是轴承的;体细胞枝条形成由间隙连接耦合的第二网络,而轴突端子(ATS)在外层络合物层(OPL)中形成第三独立网络。小鼠视网膜仅具有一种类型的HC,其形态学类似于兔的B型HC。以前的工作表明,鼠标HCS Express CX57(J Neurosci eur 19:2633-2640)。因此,我们克隆了兔CX57并提出了一种抗体,以确定兔HC之间CX57间隙连接的分布。使用染料注射方法用于通过共聚焦显微镜进行分析的所有三个HC网络的详细填充。我们发现CX57与Plexus的B型相关。 CX57斑块与B型躯体树突和A型HC网络吻合。此外,CX50和CX57之间没有分致化。我们得出结论,在兔视网膜中,CX57仅在BHC过程中发现。因此,在存在两种类型的HC的物种中,表示不同的连接素。在B型HCS的体细胞枝曲体中没有CX57标记表明兔中另外未识别的HC Connexin的可能性。

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