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Manipulation of 3D Cluster Size and Geometry by Release from 2D Micropatterns

机译：通过从2D MicroOpatterns释放进行3D簇大小和几何的操纵

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摘要

A novel method to control three-dimensional cell cluster size and geometry using two-dimensional patterning techniques is described. Cells were first cultured on two-dimensional micropatterned collagen using conventional soft lithography techniques. Collagenase was used to degrade the micropatterned collagen and release cells from the micropatterns, forming clusters of cells which were then resuspended in a three-dimensional collagen matrix. This method facilitated the formation of uniformly sized clusters within a single sample. By systematically varying the geometry of the two-dimensional micropatterned islands, final cluster size and cell number in three dimensions could be controlled. Using this technique, we showed that proliferation of cells within collagen gels depended on the size of clusters, suggesting an important role for multicellular structure on biological function. Furthermore, by utilizing more complex two-dimensional patterns, non-spherical structures could be produced. This technique demonstrates a simple way to exploit two-dimensional micro-patterning in order to create complex and structured multicellular clusters in a three-dimensional environment.
机译：描述了一种用二维图案化技术控制三维小区簇大小和几何的新方法。首先使用常规柔软的光刻技术首先在二维微图案胶原蛋白上培养细胞。使用胶原酶从微米图中降解微透明理由的胶原和释放细胞，形成在三维胶原基质中重悬浮的细胞簇。该方法促进了在单个样品中形成均匀大小的簇。通过系统地改变二维微图案的几何形状，可以控制三维的最终簇大小和单元数。使用这种技术，我们表明胶原凝胶中细胞的增殖依赖于簇的大小，表明多细胞结构对生物学功能的重要作用。此外，通过利用更复杂的二维图案，可以产生非球形结构。该技术演示了利用二维微图案化的简单方法，以便在三维环境中产生复杂和结构化的多细胞簇。

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