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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Effects of Gaseous CO2 and bicarbonate on the growth of Neisseria gonorrhoeae.
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Effects of Gaseous CO2 and bicarbonate on the growth of Neisseria gonorrhoeae.

机译：气态二氧化碳和碳酸氢盐对淋病奈瑟菌生长的影响。

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The quantitative and qualitative growth response of Neisseria gonorrhoeae strains was assessed under the following conditions: incubation in a candle jar (approximately 2.5% ambient CO2) on medium without bicarbonate, incubation in air on medium without bicarbonate, incubation in air on medium with bicarbonate, and incubation in air in a sealed jar on medium with bicarbonate. Incubation in the presence of ambient CO2 (candle jar) resulted in the highest plating efficiencies for the three laboratory strains 9, 62, and 2686. The addition of NaHCO3 to the medium enhanced the growth response in air of all three strains, particularly if incubation was carried out in a closed environment (sealed jar). The qualitative growth response of 34 clinical isolates and laboratory strains was assessed under the same conditions of incubation after the plating of an inoculum containing approximately 2 X 10(6) bacteria. The strains were divided into different classes based on their growth responses. About 40% of the strains grew as well on bicarbonate-containing medium incubated in air in sealed jars as they did on medium without bicarbonate incubated in a candle jar. Ten percent of the strains showed only slight growth on bicarbonate containing medium incubated in sealed jars and appeared to have an almost obligate requirement for ambient CO2. Twenty percent of the strains apparently had partially lost their requirement for gaseous C02 and showed slight growth in air on medium without bicarbonate and slight to moderate growth in air on medium containing NaHCO3.The remaining 30% seemed to have completely lost their requirement for gaseous C02 and/or the bicarbonate anion and grew almost as well in air on medium without bicarbonate as they did in either ambient C02 (candle jar) or on medium containing bicarbonate incubated in a sealed jar. These results suggest that N. gonorrhoeae strains may vary widely in their requirements for CO2 and/or the HCO3-anion. Incubation in the presence of ambient CO2 tends to maximize the growth response on solid medium of those strains, which require it for growth. The presence of ambient CO2 is particularly important if growth is to be obtained after the plating of small inocula. Medium conatining 0.1% NaHCO3, if incubated in a closed environment (sealed jar), apperas to be equivalent to medium without bicarbonate incubated in ambient CO2 in supporting the growth of some but not all strains of N. gonorrhoeae.
机译：在以下条件下评估淋病奈瑟氏球菌菌株的定量和定性生长反应：在不含碳酸氢盐的培养基中于烛罐中孵育（约2.5％的环境CO2），在不含碳酸氢盐的培养基中于空气中孵育，在含碳酸氢盐的培养基中于空气中孵育，并在装有碳酸氢盐的密封罐中于空气中孵育。在环境CO2（蜡烛罐）的存在下孵育会导致三种实验室菌株9、62和2686的最高平板接种效率。向培养基中添加NaHCO3会增强所有三种菌株在空气中的生长响应，尤其是在孵育时在密闭环境（密封罐）中进行。在接种约2 X 10（6）细菌的接种物后，在相同的孵育条件下评估了34种临床分离株和实验室菌株的定性生长反应。根据菌株的生长反应将其分为不同的类别。大约40％的菌株在密封罐中在空气中孵育的含碳酸氢盐的培养基上也生长得很好，就像在烛瓶中未孵育碳酸氢盐的培养基上生长一样。 10％的菌株在密封罐中温育的含碳酸氢盐的培养基上仅表现出轻微的生长，并且几乎需要吸收周围的二氧化碳。 20％的菌株显然部分失去了对气态CO2的需求，在没有碳酸氢盐的培养基上空气中显示出轻微的增长，而在含有NaHCO3的介质中空气中显示出中度增长，其余30％似乎完全失去了对气态CO2的显示。和/或碳酸氢根阴离子在空气中在没有碳酸氢根的培养基中的生长几乎与在环境CO2（蜡烛罐）中或在密封罐中孵育的含有碳酸氢根的培养基中的生长一样好。这些结果表明淋病奈瑟氏球菌菌株对CO 2和/或HCO 3-阴离子的需求可能差异很大。在周围二氧化碳的存在下进行培养往往会使那些菌株在固体培养基上的生长反应最大化，而这需要生长。如果要在接种小接种物后获得生长，则环境CO2的存在尤其重要。如果在封闭环境（密封罐）中孵育，包含0.1％NaHCO3的培养基应等同于在环境CO2中孵育不含碳酸氢盐的培养基，以支持部分而非全部淋病奈瑟氏球菌的生长。

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