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Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles

机译:胶体金纳米颗粒与人血的相互作用:对粒径的影响和血浆蛋白结合谱的分析

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摘要

Nanoparticle size and plasma binding profile contribute to a particle’s longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.
机译:纳米粒子的大小和血浆结合特性有助于粒子在血液中的寿命,这对治疗效果可能会产生重要影响。在这项研究中,在人血浆中体外孵育30和50 nm胶体金后,观察到纳米颗粒的流体动力学尺寸大约增加了一倍。已经确定了结合柠檬酸盐稳定的金胶体表面的血浆蛋白。已经研究了蛋白质结合对纳米颗粒流体动力学大小,凝血元素和补体系统的影响。还讨论了从动态光散射,电子显微镜和扫描探针显微镜获得的尺寸测量结果的差异。

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