Heptakis(26-O-dimethyl)beta-cyclodextrin: a novel growth stimulant for Bordetella pertussis phase I.

机译：庚（26-O-二甲基）β-环糊精：百日咳博德特氏菌一期的新型生长刺激剂。

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The effect of cyclodextrins on the growth of Bordetella pertussis Tohama phase I in synthetic medium was evaluated. The addition of cyclodextrins, especially heptakis(2,6-O-dimethyl)beta-cyclodextrin (Me beta CD), to a complete synthetic medium such as Stainer-Scholte medium gave the same number of individual colonies and growth rates as those on Bordet-Gengou medium. Furthermore, with the addition of Me beta CD, growth inhibition by fatty acids such as oleic or palmitic acid was overcome and normal cell growth was observed. This modified Stainer-Scholte medium, designated as cyclodextrin solid medium (CSM), supported excellent growth of 20 lyophilized clinical isolates. Serotypes of the organisms after 10 passages on this CSM plate were not changed. These results suggest that Me beta CD is a significant growth stimulant and CSM is one of the most suitable synthetic media for culture of B. pertussis phase I.

机译：评价了环糊精对百日咳博德特氏菌Tohama I期在合成培养基中生长的影响。将环糊精，特别是庚七（2,6-O-二甲基）β-环糊精（Me beta CD）添加到完整的合成培养基（例如Stainer-Scholte培养基）中，可以得到与Bordet相同的菌落数量和生长速率-根沟培养基。此外，通过添加MeβCD，克服了脂肪酸（如油酸或棕榈酸）的生长抑制作用，并观察到正常的细胞生长。这种改良的Stainer-Scholte培养基（称为环糊精固体培养基（CSM））支持20种冻干临床分离株的出色生长。在该CSM板上经过10次传代后，微生物的血清型没有改变。这些结果表明，MeβCD是一种重要的生长刺激剂，而CSM是百日咳博德特氏菌I期培养最合适的合成培养基之一。

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期刊名称 Journal of Clinical Microbiology
作者
A Imaizumi; Y Suzuki; S Ono; H Sato; Y Sato;
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作者单位

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年(卷),期 1983(17),5
年度 1983
页码 781–786
总页数 6
原文格式 PDF
正文语种
中图分类微生物学;
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1. Heptakis(2,6-O-dimethyl)beta-cyclodextrin: a novel growth stimulant for Bordetella pertussis phase I. [J] . A Imaizumi, Y Suzuki, S Ono, Journal of Clinical Microbiology . 1983,第5期

机译：庚（2,6-O-二甲基）β-环糊精：百日咳博德特氏菌一期的新型生长刺激剂。
2. Effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin on the production of pertussis toxin by Bordetella pertussis. [J] . A Imaizumi, Y Suzuki, S Ono, Infection and immunity . 1983,第3期

机译：七（2,6-O-二甲基）β-环糊精对百日咳博德特氏菌产生百日咳毒素的影响。
3. Growth Phase- and Nutrient Limitation-Associated Transcript Abundance Regulation in Bordetella pertussis [J] . Mari M. Nakamura, Sin-Yee Liew, Craig A. Cummings, Infection and immunity . 2006,第10期

机译：百日咳博德特氏菌与生长期和营养限制相关的转录本丰度调节
4. ENGINEERING THE ADENYLATE CYCLASE TOXIN FOR USE AS A BORDETELLA PERTUSSIS VACCINE ANTIGEN [C] . Andrea M. DiVenere Biochemical and molecular engineering XX: the next generation of biochemical engineering: from nanoscale to industrial scale . 2017

机译：工程化腺苷酸环化酶毒素，用作百日咳百日咳疫苗抗原
5. Construction, mucosal immunogenicity and in vitro protective efficacy of a genetically engineered pertussis toxin-cholera toxin chimeric protein against Bordetella pertussis. [D] . Salloum, Danny F. 2001

机译：基因工程百日咳毒素-霍乱毒素嵌合蛋白针对百日咳博德特氏菌的构建，粘膜免疫原性和体外保护功效。
6. Effect of heptakis (26-O-dimethyl) beta-cyclodextrin on the production of pertussis toxin by Bordetella pertussis. [O] . A Imaizumi, Y Suzuki, S Ono, 1983

机译：七（26-O-二甲基）β-环糊精对百日咳博德特氏菌产生百日咳毒素的影响。
7. Growth Phase- and Nutrient Limitation-Associated Transcript Abundance Regulation in Bordetella pertussis† [O] . Nakamura, Mari M., Liew, Sin-Yee, Cummings, Craig A., 2006

机译：百日咳博德特氏菌的生长期和营养限制相关的转录本丰度†

Heptakis(26-O-dimethyl)beta-cyclodextrin: a novel growth stimulant for Bordetella pertussis phase I.

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