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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Rapid detection of cytomegalovirus by fluorescent monoclonal antibody staining and in situ DNA hybridization in a dram vial cell culture system.
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Rapid detection of cytomegalovirus by fluorescent monoclonal antibody staining and in situ DNA hybridization in a dram vial cell culture system.

机译：通过荧光单克隆抗体染色和德拉姆瓶细胞培养系统中的原位DNA杂交快速检测巨细胞病毒。

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By using dram vial cell culture methods, three commercially available tests for cytomegalovirus (CMV) detection were compared: direct fluorescent monoclonal antibody staining for CMV-specific early and late antigens (direct FA), indirect fluorescent monoclonal antibody staining for a CMV-specific early antigen (indirect FA), and in situ DNA hybridization with a biotinylated CMV-specific DNA probe kit (DNA probe). Of those tests, only the indirect FA provided consistent, reliable virus detection within the initial 24 h postinfection for serial 10-fold dilutions of CMV AD169 (laboratory strain) and for three selected urine samples. However, when used prospectively, the indirect FA failed to detect virus within the initial 10 days postinfection in 15 of 78 consecutive specimens that were eventually positive by cell culture. Although the indirect FA was more sensitive than the direct FA or DNA probe, its utility appeared limited to specimens with high CMV concentrations. On the basis of these data, we recommend that indirect FA be reserved as an adjunct to standard cell culture for selected samples in diagnostic hospital laboratories.
机译：通过使用dram小瓶细胞培养方法，比较了三种市售巨细胞病毒（CMV）检测的测试：对CMV特异性早期和晚期抗原的直接荧光单克隆抗体染色（对FA），对CMV特异性早期的间接荧光单克隆抗体染色抗原（间接FA），并与生物素化的CMV特异性DNA探针试剂盒（DNA探针）进行原位DNA杂交。在那些测试中，对于CMV AD169（实验室菌株）的10倍系列稀释液和三个选定的尿液样本，在感染后的最初24小时内，仅间接FA提供了一致，可靠的病毒检测。但是，当前瞻性使用时，间接FA不能在感染后最初的10天内在78个连续样本中的15个样本中检测出病毒，这些样本最终通过细胞培养呈阳性。尽管间接FA比直接FA或DNA探针更敏感，但其实用性似乎仅限于高CMV浓度的标本。根据这些数据，我们建议保留间接FA作为诊断医院实验室中所选样本的标准细胞培养的辅助。

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