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Integration of 18O Labeling and Solution Isoelectric Focusing in a Shotgun Analysis of Mitochondrial Proteins

机译：O弹枪分析线粒体蛋白中18O标记和溶液等电聚焦的集成

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摘要

Forward and reverse ¹⁸O labeling are integrated with solution isoelectric focusing and capillary LC-tandem mass spectrometry to evaluate a new strategy for quantitative proteomics, and to study abundance changes in mitochondrial proteins associated with drug resistance in MCF-7 human cancer cells. Galectin-3 binding protein, which is involved in apoptosis, was detected only in the resistant cell line, as a result of reverse labeling. Among 278 proteins identified, twelve were detected with abundances altered at least 2-fold.

机译：正向和反向^{18 O标记与溶液等电聚焦和毛细管LC串联质谱相结合，以评估定量蛋白质组学的新策略，并研究线粒体蛋白的丰度变化与MCF- 7种人类癌细胞。由于反向标记，仅在抗性细胞系中检测到参与凋亡的半乳凝素3结合蛋白。在鉴定的278种蛋白质中，检测到十二种，其丰度至少改变了2倍。}

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期刊名称 other
作者
Jinshan Wang; Peter Gutierrez; Nathan Edwards; Catherine Fenselau;
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作者单位

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年(卷),期 -1(6),12
年度 -1
页码 4601–4607
总页数 15
原文格式 PDF
正文语种
中图分类
关键词
comparative proteomics sup18/supO labeling reverse labeling solution isoelectric focusing galectin 3 binding protein human mitochondria drug resistance;

机译：比较蛋白质组学;sup 18 / sup O标记;反向标记;溶液等电聚焦;半乳凝素;3种结合蛋白;人线粒体;耐药性;

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专利

1. Integration of 18O Labeling and Solution Isoelectric Focusing in a Shotgun Analysis of Mitochondrial Proteins [J] . Jinshan Wang, Peter Gutierrez, Nathan Edwards Journal of proteome research . 2007,第12期

机译：线粒体蛋白of弹枪分析中18O标记和溶液等电聚焦的集成
2. Integration of O-18 labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins [J] . Wang J, Gutierrez P, Edwards N, Journal of proteome research . 2007,第12期

机译：O-18标记和溶液等电聚焦在线粒体蛋白质a弹枪分析中的集成
3. Misidentification of nitrated peptides: comments on Hong, S.J., Gokulrangan, G., Schoneich, C., 2007. Proteomic analysis of age-dependent nitration of rat cardiac proteins by solution isoelectric focusing coupled to nanoHPLC tandem mass spectrometry. Exp. Gerontol. 42, 639-651. [J] . Prokai L Experimental Gerontology . 2009,第6a7期

机译：硝化肽的错误鉴定：对Hong，S.J.，Gokulrangan，G.，Schoneich，C.，2007的评论。通过溶液等电聚焦与nanoHPLC串联质谱联用对大鼠心脏蛋白进行年龄依赖性硝化的蛋白质组学分析。经验Gerontol。 42，639-651。
4. INTEGRATION OF A PDMS/SU-8/QUARTZ MICROFLUIDIC CHIP WITH A NOVEL MACROPOROUS POLY DIMETHYLSILOXANE (PDMS) MEMBRANE FOR ISOELECTRIC FOCUSING OF PROTEINS USING WHOLE-CHANNEL IMAGING DETECTION [C] . Seyed Mostafa Shameli, Caglar Elbuken, Carolyn L. Ren, International conference on nanochannels, microchannels and minichannels;ICNMM2010 . 2011

机译：将PDMS / SU-8 / QUARTZ微流体芯片与新型大孔聚二甲基硅氧烷（PDMS）膜集成在一起，以通过全通道成像检测等离子分离蛋白质
5. Integration of oxygen-18 labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins [D] . Wang, Jinshan. 2007

机译：氧18标记和溶液等电聚焦在线粒体蛋白质a弹枪分析中的集成
6. Integration of 18O Labeling and Solution Isoelectric Focusing in a Shotgun Analysis of Mitochondrial Proteins [O] . Jinshan Wang, Peter Gutierrez, Nathan Edwards, 2015

机译：将18O标记和溶液等电聚焦整合到线粒体蛋白的鸟枪分析中
7. Electrophoresis and Isoelectric Focusing of Whole Cell and Membrane Proteins from the Extremely Halophilic Archaebacteria [R] . Stan-Lotter, Helga, Lang, Frank J., Jr., Hochstein, Lawrence I. 1989

机译：来自极端嗜盐古细菌的全细胞和膜蛋白的电泳和等电聚焦

Integration of 18O Labeling and Solution Isoelectric Focusing in a Shotgun Analysis of Mitochondrial Proteins

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