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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemiological typing of vancomycin-resistant enterococci.
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Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemiological typing of vancomycin-resistant enterococci.

机译：万古霉素耐药肠球菌流行病学分型的随机扩增多态性DNA分型与脉冲场凝胶电泳。

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摘要

Sixty vancomycin-resistant vanA mutant Enterococcus faecium (VRE) isolates, collected during a 40-month period from 48 patients hospitalized in a French Cancer Referral Center, were typed by using random amplified polymorphic DNA (RAPD), and the results were compared with those previously obtained by typing with SmaI pulsed-field gel electrophoresis (PFGE), which is currently recognized as the "gold standard." The discriminating power of RAPD typing, with seven primers and 11 combinations of primers, was tested on 18 strains, and only the most discriminating combination was further tested on the whole collection. We compared the epidemiological usefulness of RAPD typing of 60 clinical VRE isolates with that of SmaI PFGE typing. With primers AP4 and ERIC1R, RAPD generated 30 patterns versus the 36 patterns generated by SmaI PFGE. However, this did not hamper the epidemiologically correct clustering of 15 related strains and the detection of multiple colonization in nine patients. We conclude that this simple RAPD technique is well suited to the epidemiological typing of VRE and the monitoring of its nosocomial spread.
机译：在40个月内从法国癌症转诊中心住院的48名患者中收集了60株耐万古霉素的vanA突变粪肠球菌（VRE）分离株，并使用随机扩增多态性DNA（RAPD）进行了分型。以前是通过SmaI脉冲场凝胶电泳（PFGE）打字获得的，目前被认为是“金标准”。在18个菌株上测试了具有7个引物和11个引物组合的RAPD类型识别力，并且仅在整个集合中进一步测试了最具区分性的组合。我们比较了60种临床VRE分离株的RAPD分型与SmaI PFGE分型的流行病学实用性。使用引物AP4和ERIC1R，RAPD生成了30个模式，而SmaI PFGE生成了36个模式。但是，这并没有妨碍9个患者的15个相关菌株在流行病学上正确的聚类和多重定植的检测。我们得出的结论是，这种简单的RAPD技术非常适合VRE的流行病学分型和对其医院传播的监测。

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